Duicu 2013 Can J Physiol Pharmacol
|Duicu OM, Mirica SN, Gheorgheosu DE, Privistirescu AI, Fira-Mladinescu O, Muntean DM (2013) Ageing-induced decrease in cardiac mitochondrial function in healthy rats. Can J Physiol Pharmacol 91:593-600.|
Abstract: It is widely recognized that mitochondrial dysfunction is a key component of the multifactorial process of ageing. The effects of age on individual components of mitochondrial function vary across species and strains. In the present study we investigated and compared oxygen consumption, membrane potential (Δψ), the sensitivity of the mitochondrial permeability transition pore (mPTP) to calcium overload and production of reactive oxygen species (ROS) in heart mitochondria isolated from old vs. adult healthy Sprague-Dawley (SD) rats.
Respirometry studies and Δψ measurements were performed with the Oxygraph-2k (Oroboros, Austria) equipped with a tetraphenylphosphonium electrode. ROS production and calcium retention capacity (CRC) were measured spectrofluorimetrically.
Our results showed an important decline for all bioenergetic parameters for both Complex I and Complex II supported-respiration, a decreased Δψ in mitochondria energized with Complex I substrates and an increased mitochondrial ROS production in old vs. the adult group. Mitochondrial sensitivity to Ca2+-induced mtPTP opening was also increased in the old vs. adult animals. Moreover, the protective effect of cyclosporine A on mtPTP opening was significantly reduced in the old group.
Healthy ageing is associated with heart mitochondria dysfunction in Sprague Dawley rats.
• Keywords: Cyclosporine A, Heart, Ageing, Oxidative phosphorylation, Reactive oxygen species, Calcium retention capacity
• O2k-Network Lab: RO Timisoara Muntean DM
Labels: Pathology: Aging;senescence Stress:Oxidative stress;RONS Organism: Rat Tissue;cell: Heart Preparation: Isolated mitochondria Enzyme: Complex I, Complex II;succinate dehydrogenase Regulation: mt-Membrane potential
Pathway: N, S HRR: Oxygraph-2k, TPP