Dungel 2011 J Biochem Mol Toxicol

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Dungel P, Haindl S, Behling T, Mayer B, Redl H, Kozlov AV (2011) Neither nitrite nor nitric oxide mediate toxic effects of nitroglycerin on mitochondria. J Biochem Mol Toxicol 25:297-302.

» PMID: 21523859

Dungel P, Haindl S, Behling T, Mayer B, Redl H, Kozlov AV (2011) J Biochem Mol Toxicol

Abstract: It is commonly accepted that the major effect of nitroglycerin (NG) is realized through the release of nitric oxide (NO) catalyzed by aldehyde dehydrogenase-2 (ALDH2). In addition, it has been shown that NG inhibits mitochondrial respiration. The aim of this study was to clarify whether NG-mediated inhibition of mitochondrial respiration is mediated by NO. In rat liver mitochondria, NG inhibited complex-I-dependent respiration and induced reactive oxygen species (ROS) production, preferentially at complex I. Both effects were insensitive to chloral hydrate, an ALDH2 inhibitor. Nitrite, an NG intermediate, had no influence on either mitochondrial respiration or the production of ROS. NO inhibited preferentially complex I but did not elevate ROS production. Hemoglobin, an NO scavenger, and blue light had contrary effects on mitochondria inhibited by NO or NG. In summary, our data suggest that although NG induces vasodilatation via NO release, it causes mitochondrial dysfunction via an NO-independent pathway.

Keywords: Nitroglycerin, Nitrite, Nitric oxide, Mitochondria, Inhibition, ROS

O2k-Network Lab: AT Vienna Kozlov AV


Labels:

Stress:Oxidative stress;RONS  Organism: Rat  Tissue;cell: Liver  Preparation: Isolated mitochondria  Enzyme: Complex I 


HRR: Oxygraph-2k