Fu 2021 Biochem Biophys Rep

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Fu X, Chen S, Wang X, Shen Y, Zeng R, Wu Q, Lu Y, Shi J, Zhou S (2021) Dendrobium nobile Lindl. alkaloids alleviate Mn-induced neurotoxicity via PINK1/Parkin-mediated mitophagy in PC12 cells. Biochem Biophys Rep 26:100877.

» PMID: 33889759 Open Access

Fu Xiaolong, Chen Shu, Wang Xueting, Shen Yanhua, Zeng Ru, Wu Qin, Lu Yuanfu, Shi Jingshan, Zhou Shaoyu (2021) Biochem Biophys Rep

Abstract: Modern pharmacological studies have demonstrated that Dendrobium nobile Lindl. Alkaloids (DNLA), the main active ingredients of Dendrobium nobile, is valuable as an anti-aging and neuroprotective herbal medicine. The present study was designed to determine whether DNLA confers protective function over neurotoxicant manganese (Mn)-induced cytotoxicity and the mechanism involved. Our results showed that pretreatment of PC12 cells with DNLA alleviated cell toxicity induced by Mn and improved mitochondrial respiratory capacity and oxidative status. Mn treatment increased apoptotic cell death along with a marked increase in the protein expression of Bax and a decrease in the expression of Bcl-2 protein, all of which were noticeably reversed by DNLA. Furthermore, DNLA significantly abolished the decrease in protein levels of both PINK1 and Parkin, and mitigated the increased expression of autophagy marker LC3-II and accumulation of p62 caused by Mn. These results demonstrate that DNLA inhibits Mn induced cytotoxicity, which may be mediated through modulating PINK1/Parkin-mediated autophagic flux and improving mitochondrial function. Keywords: Autophagy, Dendrobium nobile Lindl. alkaloid, Manganese, PINK1, Parkin, ROS Bioblast editor: Reiswig R O2k-Network Lab: CN Zunyi Zhou S


Labels: MiParea: Respiration, Pharmacology;toxicology 


Organism: Rat  Tissue;cell: Nervous system  Preparation: Intact cells 


Coupling state: ROUTINE 

HRR: Oxygraph-2k 

2021-08