Geyer 1951 J Biol Chem
| Geyer RP, Cunningham M, Bendergast J (1951) Inhibition studies on radioactive fatty acid metabolism. J Biol Chem 188:185-91 |
Geyer RP, Cunningham M, Bendergast J (1951) J Biol Chem
Abstract: Labeled carbon dioxide and acetoacetate are two of the chief products formed during the incubation of radioactive fatty acids with rat tissue slices (1, 2). The quantitative aspects of the distribution of radiocarbon in these products can be affected by (a) the kind of tissue, (b) the length of the carbon chain of the substrate, and (c) the odd or even character of the substrate carbon chain (1). In addition, it has been shown that the amount of C1402 produced can be altered by a number of inhibitors (3). In the case of malonic acid, it was found that the decrease in total C1402 which resulted was accompanied by an increase in the Cl4 content of the carboxyl group of the acetoacetic acid formed. This finding suggested that the breakdown of fatty acids to the 2-carbon fragment stage did not depend on the operation of the intact tricarboxylic acid cycle, and, furthermore, supported the concept that the 2-carbon fragments formed were capable of following several different metabolic pathways. The nature of the data available, however, made it impossible to deduce whether one or both species of acetic acid radicals (CHSCO- and -CH&O-) were subject to this choice of pathways. Since the degree of asymmetry in the labeling of the acetoacetic acid formed involves the relative numbers of each of these species present (4, 5), information concerning the flexibility of the pathway of each type of fragment can be gained by the simultaneous study of the respired carbon dioxide and acetoacetic acid formed in the presence and absence of various inhibitors. Such studies are reported in the present paper. The experiments involved the incubation of rat liver with various labeled fatty acids in the presence of various inhibitors. Of the latter compounds, malonic acid was studied the most intensively.
• Bioblast editor: Gnaiger E
Labels:
Organism: Rat
Tissue;cell: Liver
Regulation: Inhibitor
Pathway: F, S
