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Hassan 2020 MitoFit Preprint Arch

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Hassan 2020 MitoFit Preprint Arch

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Hassan Hazirah, Gnaiger Erich, Zakaria Fazaine, Makpol Suzana, Abdul Karim Norwahidah (2020) Alterations in mitochondrial respiratory capacity and membrane potential: a link between mitochondrial dysregulation and autism.

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Alterations in mitochondrial respiratory capacity and membrane potential: a link between mitochondrial dysregulation and autism. -

Hassan Hazirah, Gnaiger Erich, Zakaria Fazaine, Makpol Suzana, Abdul Karim Norwahidah (2020) MitoFit Preprint Arch

Abstract: Version 1 (v1) 2020-04-30 doi:10.26124/mitofit:200003

Mitochondrial dysfunction has been implicated in the pathogenesis of autism. We compared mitochondrial respiratory control in autism with normal lymphoblastoid cell line and found that the oxidative phosphorylation and electron transfer of the NADH- and succinate-linked pathways, and cytochrome c oxidase activity of the autism lymphoblastoid cell line were higher. Mitochondrial membrane potential was also higher in the succinate-pathway during LEAK respiration and oxidative phosphorylation. Taken together, these results indicate abnormalities in mitochondrial function with autism. Understanding the link between mitochondrial dysfunction and autism is important for early and effective interventions.

Keywords: autism spectrum disorders, oxidative phosphorylation, cytochrome c oxidase activity, mitochondrial membrane potential, mitochondrial disease Bioblast editor: Gnaiger Erich O2k-Network Lab: MY Kuala Lumpur Abdul Karim N, AT Innsbruck Gnaiger E, AT Innsbruck Oroboros


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Labels: MiParea: Respiration, mt-Medicine  Pathology: Autism  Stress:Mitochondrial disease  Organism: Human 

Preparation: Permeabilized cells  Enzyme: Complex IV;cytochrome c oxidase  Regulation: Coupling efficiency;uncoupling, mt-Membrane potential  Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, CIV, NS, ROX  HRR: Oxygraph-2k, O2k-Fluorometer