Hort 2014 Biochimie

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Hort MA, Straliotto MR, de Oliveira J, Amoêdo ND, da Rocha JB, Galina A, Ribeiro-do-Valle RM, de Bem AF (2014) Diphenyl diselenide protects endothelial cells against oxidized low density lipoprotein-induced injury: Involvement of mitochondrial function. Biochimie 105:172-81.

» PMID: 25038571

Hort MA, Straliotto MR, de Oliveira J, Amoedo ND, da Rocha JB, Galina A, Ribeiro-do-Valle RM, de Bem AF (2014) Biochimie

Abstract: Elevated levels of oxidized low density lipoprotein (oxLDL) are considered to be one of the major risk factors for atherosclerosis and cardiovascular morbidity. The early stages of atherosclerosis are initiated by the accumulation of oxLDL and the induction of toxic effects on endothelial cells, resulting in endothelial dysfunction. The aim of this study was to investigate how diphenyl diselenide (DD), an organoselenium compound, protect vascular endothelial cells against the toxic effects of oxLDL in vitro. Our data showed that the treatment of bovine endothelial aortic cells (BAEC) with DD (0.1-1 μM) for 24 h protected from oxLDL-induced reactive species (RS) production and reduced glutathione (GSH) depletion. Moreover, DD (1 μM) per se improved the maximal mitochondrial respiratory capacity and prevented oxLDL-induced mitochondrial damage. In addition, DD could prevent apoptosis induced by oxLDL in BAEC. Results from this study may provide insight into a possible molecular mechanism underlying DD suppression of oxLDL-mediated vascular endothelial dysfunction.

Keywords: Bovine aortic endothelial cells

O2k-Network Lab: BR Rio de Janeiro Rumjanek FD, BR Rio de Janeiro Galina A


Labels: MiParea: Respiration, Pharmacology;toxicology 


Organism: Bovines  Tissue;cell: Other cell lines  Preparation: Intact cells 


Coupling state: LEAK, ROUTINE, ET 

HRR: Oxygraph-2k