Kladnicka 2021 Biomolecules

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Kladnicka I, Cedikova M, Jedlicka J, Kohoutova M, Muller L, Plavinova I, Kripnerova M, Bludovska M, Kuncova J, Mullerova D (2021) Chronic DDE exposure modifies mitochondrial respiration during differentiation of human adipose-derived mesenchymal stem cells into mature adipocytes. Biomolecules 11:1068.

» PMID: 34439734 Open Access

Kladnicka Iva, Cedikova Miroslava, Jedlicka Jan, Kohoutova Michaela, Muller Ludek, Plavinova Iveta, Kripnerova Michaela, Bludovska Monika, Kuncova Jitka, Mullerova Dana (2021) Biomolecules

Abstract: The contribution of environmental pollutants to the obesity pandemic is still not yet fully recognized. Elucidating possible cellular and molecular mechanisms of their effects is of high importance. Our study aimed to evaluate the effect of chronic, 21-day-long, 2,2-bis (4-chlorophenyl)-1,1-dichlorethylenedichlorodiphenyldichloroethylene (p,p'-DDE) exposure of human adipose-derived mesenchymal stem cells committed to adipogenesis on mitochondrial oxygen consumption on days 4, 10, and 21. In addition, the mitochondrial membrane potential (MMP), the quality of the mitochondrial network, and lipid accumulation in maturing cells were evaluated. Compared to control differentiating adipocytes, exposure to p,p'-DDE at 1 μM concentration significantly increased basal (routine) mitochondrial respiration, ATP-linked oxygen consumption and MMP of intact cells on day 21 of adipogenesis. In contrast, higher pollutant concentration seemed to slow down the gradual increase in ATP-linked oxygen consumption typical for normal adipogenesis. Organochlorine p,p'-DDE did not alter citrate synthase activity. In conclusion, in vitro 1 μM p,p'-DDE corresponding to human exposure is able to increase the mitochondrial respiration per individual mitochondrion at the end of adipocyte maturation. Our data reveal that long-lasting exposure to p,p'-DDE could interfere with the metabolic programming of mature adipocytes. Keywords: Adipogenesis, Human adipose-derived mesenchymal stem cells, Mitochondrial respiration, p, p′-DDE Bioblast editor: Plangger M O2k-Network Lab: CZ Pilsen Kuncova J


Labels: MiParea: Respiration, Pharmacology;toxicology  Pathology: Obesity 

Organism: Human  Tissue;cell: Fat  Preparation: Intact cells 


Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

2021-10