Kukat 2014 PLoS Genet
|Kukat A, Dogan SA, Edgar D, Mourier A, Jacoby C, Maiti P, Mauer J, Becker C, Senft K, Wibom R, Kudin AP, Hultenby K, Flögel U, Rosenkranz S, Ricquier D, Kunz WS, Trifunovic A (2014) Loss of UCP2 attenuates mitochondrial dysfunction without altering ROS production and uncoupling activity. https://doi.org/10.1371/journal.pgen.1004385|
» PLoS Genet 10:e1004385. PMID: 24945157 Open Access
Kukat Alexandra, Dogan Sukru Anil, Edgar Daniel, Mourier Arnaud, Jacoby Christoph, Maiti Priyanka, Mauer Jan, Becker Christina, Senft Katharina, Wibom Rolf, Kudin Alexei P, Hultenby Kjell, Floegel Ulrich, Rosenkranz Sephan, Ricquier Daniel, Kunz Wolfram S, Trifunovic Aleksandra (2014) PLoS Genet
Abstract: Although mitochondrial dysfunction is often accompanied by excessive reactive oxygen species (ROS) production, we previously showed that an increase in random somatic mtDNA mutations does not result in increased oxidative stress. Normal levels of ROS and oxidative stress could also be a result of an active compensatory mechanism such as a mild increase in proton leak. Uncoupling protein 2 (UCP2) was proposed to play such a role in many physiological situations. However, we show that upregulation of UCP2 in mtDNA mutator mice is not associated with altered proton leak kinetics or ROS production, challenging the current view on the role of UCP2 in energy metabolism. Instead, our results argue that high UCP2 levels allow better utilization of fatty acid oxidation resulting in a beneficial effect on mitochondrial function in heart, postponing systemic lactic acidosis and resulting in longer lifespan in these mice. This study proposes a novel mechanism for an adaptive response to mitochondrial cardiomyopathy that links changes in metabolism to amelioration of respiratory chain deficiency and longer lifespan.
Labels: MiParea: Respiration, mtDNA;mt-genetics
Stress:Oxidative stress;RONS Organism: Mouse Tissue;cell: Heart Preparation: Isolated mitochondria Enzyme: Uncoupling protein Regulation: mt-Membrane potential Coupling state: LEAK, OXPHOS, ET Pathway: F, N, S, NS, ROX HRR: Oxygraph-2k, O2k-Fluorometer