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Lages 2015 PeerJ

From Bioblast
Publications in the MiPMap
Lages YM, Nascimento JM, Lemos GA, Galina A, Castilho LR, Rehen SK (2015) Low oxygen alters mitochondrial function and response to oxidative stress in human neural progenitor cells. PeerJ 3:e1486.

ยป PMID: 26713239 Open Access

Lages YM, Nascimento JM, Lemos GA, Galina A, Castilho LR, Rehen SK (2015) PeerJ

Abstract: Oxygen concentration should be carefully regulated in all living tissues, beginning at the early embryonic stages. Unbalances in oxygen regulation can lead to cell death and disease. However, to date, few studies have investigated the consequences of variations in oxygen levels for fetal-like cells. Therefore, in the present work, human neural progenitor cells (NPCs) derived from pluripotent stem cells grown in 3% oxygen (v/v) were compared with NPCs cultured in 21% (v/v) oxygen. Low oxygen concentrations altered the mitochondrial content and oxidative functions of the cells, which led to improved ATP production, while reducing generation of reactive oxygen species (ROS). NPCs cultured in both conditions showed no differences in proliferation and glucose metabolism. Furthermore, antioxidant enzymatic activity was not altered in NPCs cultured in 3% oxygen under normal conditions, however, when exposed to external agents known to induce oxidative stress, greater susceptibility to DNA damage was observed. Our findings indicate that the management of oxygen levels should be considered for in vitro models of neuronal development and drug screening. โ€ข Keywords: Cell metabolism, DNA damage, Human neural progenitor cells, Low oxygen, Mitochondria, Reactive oxygen species

โ€ข O2k-Network Lab: BR Rio de Janeiro Galina A, BR Rio de Janeiro Institute Biomedical Chemistry

Labels: MiParea: Respiration, mt-Biogenesis;mt-density 

Stress:Oxidative stress;RONS, Hypoxia  Organism: Human  Tissue;cell: Stem cells  Preparation: Intact cells 

Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k