Lima 2018 Biochem Cell Biol

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Lima T, Silveira L (2018) A microplate assay for measuring cell death in C2C12 cells. Biochem Cell Biol 96:702-6.

» PMID: 29566341

Lima T, Silveira L (2018) Biochem Cell Biol

Abstract: The main goal of this study was to develop a straightforward and rapid microplate assay for measuring propidium iodide (PI) in C2C12 cells. The PI method proves to be an efficient quantitative assay for analyzing cell viability through PI fluorescence analysis. Importantly, the protocol takes less than 30 minutes, and the results are reproducible. C2C12 cells were exposed to an increasing concentration of palmitate for a period of 24 hours to induce cell death, and the PI fluorescence increased in a concentration-dependent manner. Evaluation of mitochondrial function and reactive oxygen species production validated the deleterious effects of palmitate treatment. Also, the microplate PI assay demonstrated high sensitivity as indicated by the detection of modest fluctuations in cell viability in response to catalase overexpression in palmitate-treated cells. The microplate PI assay, therefore, offers an accurate method to be used for in vitro studies.

Bioblast editor: Kandolf G

Labels: MiParea: Respiration 

Stress:Cell death  Organism: Mouse  Tissue;cell: Skeletal muscle, Other cell lines  Preparation: Intact cells 

Regulation: Fatty acid  Coupling state: LEAK, ET  Pathway:HRR: Oxygraph-2k 

Labels, 2018-04