Ljubkovic 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

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Ljubkovic 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

Link: O2k-Network Discussion Forum

Ljubkovic M (2015)

Event: Fatty Acid Oxidation O2k-Network Discussion Forum

I am sending you a graph with some mitochondrial respiration measurements obtained from the left ventricular human tissue of patients undergoing CABG surgery. As can be seen from the graph, we used FFA substrate to evoke the State 2 and State 3 respiration, and then followed with other substrates and an uncoupler. FFA substrate was palmitoyl-carnitine (40 µM concentration). We performed quite a few of experiments using palmitoyl-carnitine, also in the range of concentrations from 1 – 40 µM. We usually see a gradual increase in State 3 respiration with concentration increments (1, 2.5, 5, 10, 15, 40 µM). However, the respiration is always lower then when pyruvate is used (although in much higher concentration, from 2 mM up). All of the observations come from the human left ventricular tissue, that we almost exclusively use in past couple of years for our experiments on mitochondria (we also measured activity of some enzymes and protein expression).


O2k-Network Lab: HR Split Ljubkovic M


Labels: MiParea: Patients 


Organism: Human  Tissue;cell: Heart  Preparation: Permeabilized tissue 


Coupling state: OXPHOS, ET  Pathway: F, N, NS 


Palmitoylcarnitine 

Figure

Jjubkovic 2015 Fatty Acid Oxidation O2k-Network Discussion Forum Figure.jpg
We performed mitochondrial respiration measurement in permeabilized skinned fibres from human left ventricle biopsies obtained from 20 patients. Panel A displays the recorded values of oxygen consumption normalized per wet tissue weight, while panel B presents the respiration values relative to maximal mitochondrial respiration (evoked by addition of substrates for all complexes and an uncoupler). Arrows indicate addition of specific substrates during a respiration recording protocol. Pc=palmitoylcarnitine (40 μM), M=malate (5 mM), P=pyruvate (10 mM), G=glutamate (10 mM), S=succinate (15 mM), ADP 2.5 mM, FCCP 1 μM.