Llobet 2015 Dis Model Mech

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Llobet L, Toivonen JM, Montoya J, Ruiz-Pesini E, López-Gallardo E (2015) OXPHOS xenobiotics alter adipogenic differentiation at concentrations found in human blood. Dis Model Mech 8:1441-55.

» PMID: 26398948

Llobet L, Toivonen JM, Montoya J, Ruiz-Pesini E, Lopez-Gallardo E (2015) Dis Model Mech

Abstract: Adipogenesis is accompanied by differentiation of adipose tissue-derived stem cells to adipocytes. As a part of this differentiation, biogenesis of the oxidative phosphorylation system occurs. Many chemical compounds used in medicine, agriculture, or other human activities, affect the oxidative phosphorylation function. Therefore, these xenobiotics could alter adipogenesis. We have analyzed the effects on adipocyte differentiation of some xenobiotics that act on the oxidative phosphorylation system. The tested concentrations have been previously reported in human blood. Our results show that pharmaceutical drugs that decrease mitochondrial DNA replication, such as nucleoside reverse transcriptase inhibitors, or inhibitors of mitochondrial protein synthesis, like ribosomal antibiotics, diminish adipocyte differentiation and leptin secretion. On the other hand, the environmental chemical pollutant tributyltin chloride, inhibiting the ATP synthase of the oxidative phosphorylation system, can promote adipocyte differentiation and leptin secretion, leading to obesity and metabolic syndrome as the obesogen hypothesis postulates.

Keywords: OXPHOS, Xenobiotics, Adipocytes, Human adipose tissue-derived stem cells

O2k-Network Lab: ES Zaragoza Ruiz-Pesini E

Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Pharmacology;toxicology 

Organism: Human  Tissue;cell: Fat, Stem cells  Preparation: Intact cells  Enzyme: Complex II;succinate dehydrogenase, Complex IV;cytochrome c oxidase 

Coupling state: LEAK, ROUTINE, ET 

HRR: Oxygraph-2k