Mastronicola 2011 IUBMB Life
|Mastronicola D, Giuffrè A, Testa F, Mura A, Forte E, Bordi E, Pucillo LP, Fiori PL, Sarti P (2011) Giardia intestinalis escapes oxidative stress by colonizing the small intestine: A molecular hypothesis. IUBMB Life 63:21-5.|
Abstract: Giardia intestinalis is the microaerophilic protozoon causing giardiasis, a common infectious intestinal disease. Giardia possesses an O2 -scavenging activity likely essential for survival in the host. We report that Giardia trophozoites express the O2 -detoxifying flavodiiron protein (FDP), detected by immunoblotting, and are able to reduce O2 to H2O rapidly (∼3 μM O2 × min × 106 cells at 37 °C) and with high affinity (C50 = 3.4 ± 0.7 μM O2). Following a short-term (minutes) exposure to H2O2 ≥ 100 μM, the O2 consumption by the parasites is irreversibly impaired, and the FDP undergoes a degradation, prevented by the proteasome-inhibitor MG132. Instead, H2O2 does not cause degradation or inactivation of the isolated FDP. On the basis of the elevated susceptibility of Giardia to oxidative stress, we hypothesize that the parasite preferentially colonizes the small intestine since, compared with colon, it is characterized by a greater capacity for redox buffering and a lower propensity to oxidative stress.
• Keywords: Giardia intestinalis, Oxygen consumption, Flavoprotein, Detoxifying enzyme, Oxidative stress, Cell proteolysis, Protozoan pathogen
• O2k-Network Lab: IT Rome Sarti P
Stress:Oxidative stress;RONS Organism: Protists
Preparation: Intact cells