Napa 2017 Int J Dent

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Napa K, Baeder AC, Witt JE, Rayburn ST, Miller MG, Dallon BW, Gibbs JL, Wilcox SH, Winden DR, Smith JH, Reynolds PR, Bikman BT (2017) LPS from P. gingivalis negatively alters gingival cell mitochondrial bioenergetics. Int J Dent 2017:2697210.

» Open Access

Napa K, Baeder AC, Witt JE, Rayburn ST, Miller MG, Dallon BW, Gibbs JL, Wilcox SH, Winden DR, Smith JH, Reynolds PR, Bikman BT (2017) Int J Dent

Abstract: Oral inflammatory pathologies are linked to increased oxidative stress, thereby partly explaining their relevance in the etiology of systemic disorders. The purpose of this work was to determine the degree to which LPS from Porphyromonas gingivalis, the primary pathogen related to oral inflammation, altered gingival mitochondrial function and reactive oxygen species generation.

Human gingival fibroblast (HGF-1) cells were treated with lipopolysaccharide of P. gingivalis. Mitochondrial function was determined via high-resolution respirometry.

LPS-treated HGF-1 cells had significantly more mitochondrial complex IV and higher rates of mitochondrial respiration. However, this failed to translate into greater ATP production, as ATP production was paradoxically diminished with LPS treatment. Nevertheless, production of the reactive H2O2 was elevated with LPS treatment.

LPS elicits an increase in gingival cell mitochondria content, with a subsequent increase in reactive oxygen species production (i.e. H2O2), despite a paradoxical reduction in ATP generation. These findings provide insight into the nature of oxidative stress in oral inflammatory pathologies.

Keywords: Mitochondria, Respiration, LPS, Oral gingiva, Oxidative stress; HGF-1 human gingival fibroblast Bioblast editor: Kandolf G O2k-Network Lab: US UT Provo Bikman BT


Labels: MiParea: Respiration, Pharmacology;toxicology 


Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell  Preparation: Permeabilized cells 

Regulation: ATP production  Coupling state: LEAK, OXPHOS, ET  Pathway: N, NS, ROX  HRR: Oxygraph-2k, O2k-Fluorometer 

2017-06, Magnesium Green, Amplex UltraRed