Nascimento-Dos-Santos 2020 Biochim Biophys Acta Mol Basis Dis
|Nascimento-Dos-Santos G, de-Souza-Ferreira E, Lani R, de Faria CC, Araujo VG, Teixeira-Pinheiro LC, de Souza Vasconcelos T, Leandro TG, Santiago MF, Linden R, Galina A, Petrs-Silva H (2020) Neuroprotection from optic nerve injury and modulation of oxidative metabolism by transplantation of active mitochondria to the retina. Biochim Biophys Acta Mol Basis Dis 1866:165686.|
Nascimento-Dos-Santos G, de-Souza-Ferreira E, Lani R, de Faria CC, Araujo VG, Teixeira-Pinheiro LC, de Souza Vasconcelos T, Leandro TG, Santiago MF, Linden R, Galina A, Petrs-Silva H (2020) Biochim Biophys Acta Mol Basis Dis
Abstract: Mitochondrial dysfunctions are linked to a series of neurodegenerative human conditions, including Parkinson's disease, schizophrenia, optic neuropathies, and glaucoma. Recently, a series of studies have pointed mitotherapy - exogenous mitochondria transplant - as a promising way to attenuate the progression of neurologic disorders; however, the neuroprotective and pro-regenerative potentials of isolated mitochondria in vivo have not yet been elucidated. In this present work, we tested the effects of transplants of active (as well-coupled organelles were named), liver-isolated mitochondria on the survival of retinal ganglion cells and axonal outgrowth after optic nerve crush. Our data show that intravitreally transplanted, full active mitochondria incorporate into the retina, improve its oxidative metabolism and electrophysiological activity at 1 day after transplantation. Moreover, mitotherapy increases cell survival in the ganglion cell layer at 14 days, and leads to a higher number of axons extending beyond the injury site at 28 days; effects that are dependent on the organelles' structural integrity. Together, our findings support mitotherapy as a promising approach for future therapeutic interventions upon central nervous system damage.
Copyright © 2020. Published by Elsevier B.V.
• Keywords: Active mitochondria, CNS neuroprotection, Glaucoma, Mitochondria transplantation, Mitotherapy, Optic nerve crush • Bioblast editor: Plangger M • O2k-Network Lab: BR Rio de Janeiro Galina A, BR Rio de Janeiro Institute Biomedical Chemistry
Labels: MiParea: Respiration, mt-Medicine Pathology: Neurodegenerative
Organism: Rat Tissue;cell: Nervous system, Liver Preparation: Isolated mitochondria, Intact cells
Coupling state: LEAK, ROUTINE, OXPHOS, ET Pathway: N, S, NS, ROX HRR: Oxygraph-2k