Papadimitriou 2019 Sci Rep

From Bioblast
Publications in the MiPMap
Papadimitriou ID, Eynon N, Yan X, Munson F, Jacques M, Kuang J, Voisin S, North KN, Bishop DJ (2019) A "human knockout" model to investigate the influence of the Ξ±-actinin-3 protein on exercise-induced mitochondrial adaptations. Sci Rep 9:12688.

Β» PMID: 31481717 Open Access

Papadimitriou ID, Eynon N, Yan X, Munson F, Jacques M, Kuang J, Voisin S, North KN, Bishop DJ (2019) Sci Rep

Abstract: Research in Ξ±-actinin-3 knockout mice suggests a novel role for Ξ±-actinin-3 as a mediator of cell signalling. We took advantage of naturally-occurring human "knockouts" (lacking Ξ±-actinin-3 protein) to investigate the consequences of Ξ±-actinin-3 deficiency on exercise-induced changes in mitochondrial-related genes and proteins, as well as endurance training adaptations. At baseline, we observed a compensatory increase of Ξ±-actinin-2 protein in ACTN3 XX (Ξ±-actinin-3 deficient; n = 18) vs ACTN3 RR (expressing Ξ±-actinin-3; n = 19) participants but no differences between genotypes for markers of aerobic fitness or mitochondrial content and function. There was a main effect of genotype, without an interaction, for RCAN1-4 protein content (a marker of calcineurin activity). However, there was no effect of genotype on exercise-induced expression of genes associated with mitochondrial biogenesis, nor post-training physiological changes. In contrast to results in mice, loss of Ξ±-actinin-3 is not associated with higher baseline endurance-related phenotypes, or greater adaptations to endurance exercise training in humans.

β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: AU Melbourne Stepto NK

Labels: MiParea: Respiration, Genetic knockout;overexpression, Exercise physiology;nutrition;life style 

Organism: Human  Tissue;cell: Skeletal muscle  Preparation: Permeabilized tissue 

Coupling state: LEAK, OXPHOS, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k 

Labels, 2019-09 

Cookies help us deliver our services. By using our services, you agree to our use of cookies.