Smenes 2015 Abstract IOC100

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Smenes BT, Baekkerud FH, Hassel E, Wohlwend M, Slagsvold KH, Rognmo O, Wahba A (2015) Mitochondrial function in patients exercising prior to coronary surgery – A single blinded randomized controlled trial. Mitochondr Physiol Network 20.01.

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Smenes BT, Baekkerud FH, Hassel E, Wohlwend M, Slagsvold KH, Rognmo O, Wahba A (2015)

Event: IOC100

During open heart surgery, the myocardium suffers from ischemia-reperfusion injury (IR) despite perioperative cardioprotection. This type of injury impairs mitochondrial function and leads to cell death, which impairs cardiac function and negatively affects patient outcome [1]. We previously demonstrated that remote ischemic preconditioning preserves mitochondrial function during cardiac surgery [2,3], and animal studies have shown that as little as a few bouts of endurance exercise can protect the heart from IR [4]. Up until now, exercise as preconditioning has not been studied in humans in a clinical setting.

20 patients undergoing elective isolated primary coronary artery bypass surgery at St. Olav’s University Hospital, Norway will be included. The patients are randomized to either one bout of incremental treadmill running 24 hours preoperatively (n=10), or to prepare for surgery according to standard procedures (n=10). Atrial and ventricular biopsies will be collected at two time points: Before and after aortic cross-clamping during extracorporeal circulation. The Oxygraph-2k (Oroboros Instruments, Innsbruck, Austria) will be used to assess mitochondrial respiration and the fluorescence module allows us to measure H2O2 production. A substrate-uncoupler-inhibitor-titration protocol will be used to assess different aspects of integrated TCA cycle function and electron transport system.

Due to blinding, we are not able to present any preliminary data before data collection is completed. We hypothesize that one bout of exercise one day prior to open heart surgery is sufficient to induce cardioprotection and preserve mitochondrial function.


O2k-Network Lab: NO Trondheim Rognmo O


Labels: MiParea: Respiration, Exercise physiology;nutrition;life style, Patients  Pathology: Cardiovascular  Stress:Ischemia-reperfusion  Organism: Human  Tissue;cell: Skeletal muscle 


Coupling state: LEAK, ROUTINE, ET  Pathway: N, S  HRR: Oxygraph-2k, O2k-Fluorometer 


Affiliations

Inst Circulation Med Imaging, Norwegian Univ Science Technology (NTNU), Norway. - btsmenes@gmail.com

References

  1. Anselmi A, Abbate A, Girola F, Nasso G, Biondi-Zoccai GG, Possati G, Gaudino M (2004) Myocardial ischemia, stunning, inflammation, and apoptosis during cardiac surgery: a review of evidence. Eur J Cardiothorac Surg 25:304-11.
  2. Slagsvold KH, Rognmo O, Hoydal M, Wisloeff U, Wahba A (2014) Remote ischemic preconditioning preserves mitochondrial function and influences myocardial microrna expression in atrial myocardium during coronary bypass surgery. Circ Res 114:851-9.
  3. Slagsvold KH, Moreira JBN, Rognmo O, Hoydal MA, Bye A, Wisloeff U, Wahba A (2014) Remote ischemic preconditioning preserves mitochondrial function and activates pro-survival protein kinase Akt in the left ventricle during cardiac surgery: A randomized trial. Int J Cardiol 177:409-17
  4. Powers SK, Quindry JC, Kavazis AN (2008) Exercise-induced cardioprotection against myocardial ischemia-reperfusion injury. Free Radic Biol Med 44:193-201.