Smith 2020 J Biol Chem

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Publications in the MiPMap
Smith CD, Schmidt CA, Lin CT, Fisher-Wellman KH, Neufer PD (2020) Flux through mitochondrial redox circuits linked to nicotinamide nucleotide transhydrogenase generates counterbalance changes in energy expenditure. J Biol Chem 295:16207-16.

Β» PMID: 32747443 Open Access

Smith Cody D, Schmidt Cameron A, Lin Chien-Te, Fisher-Wellman Kelsey H, Neufer P Darrell (2020) J Biol Chem

Abstract: Compensatory changes in energy expenditure occur in response to positive and negative energy balance, but the underlying mechanism remains unclear. Under low energy demand, the mitochondrial electron transport system (ETS) is particularly sensitive to added energy supply (i.e., reductive stress) which exponentially increases the rate of H2O2 (JH2O2) production. H2O2 is reduced to H2O by electrons supplied by NADPH. NADP+ is reduced back to NADPH by activation of mitochondrial membrane potential-dependent nicotinamide nucleotide transhydrogenase (NNT). The coupling of reductive stress-induced JH2O2 production to NNT-linked redox buffering circuits provides a potential means of integrating energy balance with energy expenditure. To test this hypothesis, energy supply was manipulated by varying flux rate through Ξ²-oxidation in muscle mitochondria minus/plus pharmacological or genetic inhibition of redox buffering circuits. Here we show during both non-ADP and low-ADP stimulated respiration that accelerating flux through Ξ²-oxidation generates a corresponding increase in mitochondrial JH2O2 production, that the majority (∼70-80%) of H2O2 produced is reduced to H2O by electrons drawn from redox buffering circuits supplied by NADPH, and that the rate of electron flux through redox buffering circuits is directly linked to changes in oxygen consumption mediated by NNT. These findings provide evidence that redox reactions within Ξ²-oxidation and the ETS serve as a barometer of substrate flux relative to demand, continuously adjusting JH2O2 production and, in turn, the rate at which energy is expended via NNT-mediated proton conductance. This variable flux through redox circuits provides a potential compensatory mechanism for fine-tuning energy expenditure to energy balance in real-time. β€’ Keywords: Beta-oxidation, Bioenergetics, Electron transport system (ETS), Energy metabolism, Hydrogen sulfide, Mitochondrial metabolism, Nicotinamide nucleotide transhydrogenase, Redox regulation β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: US NC Greenville Neufer PD

Labels: MiParea: Respiration 

Stress:Oxidative stress;RONS  Organism: Mouse  Tissue;cell: Skeletal muscle  Preparation: Isolated mitochondria 

Regulation: mt-Membrane potential  Coupling state: LEAK, OXPHOS, ET  Pathway: F, N  HRR: Oxygraph-2k, O2k-Fluorometer, TPP 

2020-08, AmR 

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