Sparagna 2016 Abstract IOC109

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Sparagna GC (2016) Measuring cardiac mitochondrial function in pediatric patients before and after implantation of a left ventricular assist device. Mitochondr Physiol Network 21.01.

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Sparagna GC (2016)

Event: IOC109

Heart failure is a multifaceted disease involving both energetic and mechanical deficits. In order to help the heart mechanically, a left ventricular assist device (LVAD) is often employed in heart failure patients. This device unloads the heart and patients usually see improvements in several functional parameters. In this study, the cardiac mitochondrial function of two pediatric patients with LVADs was assessed before insertion of the LVAD and then in the explanted heart after the patients received a heart transplant.

A small piece of LV tissue was recovered at the time of LVAD implantation for both patients. Later, when these patients received a transplant, a sample from the explanted heart was used for this study. In both instances, heart tissue was rapidly harvested in the operating room, placed in BIOPS solution, kept at 4°C, and mitochondrial respirometry by Oroboros Oxygraph-2k was performed.

In the first patient, a 14.3 year old female with an LVAD for 7 days, O2k respirometry in BIOPS preserved tissue fibers showed a trend for increased respiratory control ratio (p=0.07), but no improvements in the other measured parameters using pyruvate/malate, glutamate or succinate as substrates. In contrast, the second patient, a 16 year old female, had her LVAD for 45 days and did show significant improvements in respiration rate with pyruvate/malate as well as glutamate. Respiration with succinate and the respiratory control ratio in the second patient also showed a trend for increase (both p=0.06) with the LVAD.

In summary, mitochondrial function can be measured in small pieces of heart tissue extracted during LVAD implantation. Although the LVAD is a mechanical device, its implantation results in an energetic improvement with a positive effect on mitochondrial function. This mitochondrial improvement, however, seems to take longer than a week to occur in a measurable manner.


O2k-Network Lab: US CO Aurora Sparagna GC


Labels: MiParea: Respiration, Instruments;methods, Patients  Pathology: Aging;senescence, Cardiovascular 

Organism: Human  Tissue;cell: Heart 


Coupling state: LEAK, OXPHOS  Pathway: N, S, NS  HRR: Oxygraph-2k 


Affiliation

Univ Colorado, Anschutz Med Center, Colorado, US. - sparagna@colorado.edu