Walsh 2023 FASEB J

From Bioblast
Publications in the MiPMap
Walsh MA, Musci RV, Jacobs RA, Hamilton KL (2023) A practical perspective on how to develop, implement, execute, and reproduce high-resolution respirometry experiments: The physiologist's guide to an Oroboros O2k. https://doi.org/10.1096/fj.202301644rr

Β» FASEB J 37:e23280. PMID: 37899680 Open Access

Walsh Maureen A, Musci Robert V, Jacobs Robert A, Hamilton Karyn L (2023) FASEB J

Abstract: The development of high-resolution respirometry (HRR) has greatly expanded the analytical scope to study mitochondrial respiratory control relative to specific tissue/cell types across various metabolic states. Specifically, the Oroboros Oxygraph 2000 (O2k) is a common tool for measuring rates of mitochondrial respiration and is the focus of this perspective. The O2k platform is amenable for answering numerous bioenergetic questions. However, inherent variability with HRR-derived data, both within and amongst users, can impede progress in bioenergetics research. Therefore, we advocate for several vital considerations when planning and conducting O2k experiments to ultimately enhance transparency and reproducibility across laboratories. In this perspective, we offer guidance for best practices of mitochondrial preparation, protocol selection, and measures to increase reproducibility. The goal of this perspective is to propagate the use of the O2k, enhance reliability and validity for both new and experienced O2k users, and provide a reference for peer reviewers. β€’ Keywords: Oroboros O2k, Bioenergetics, Isolated mitochondria, Mitochondria, Permeabilized fibers, Protocol development β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: US CO Colorado Springs Jacobs RA, US CO Fort Collins Hamilton K

Labels: MiParea: Respiration 

HRR: Oxygraph-2k 


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