Xia 2016 RSC Adv
|Xia Z, Yi X, Zhuang Y (2016) Stable over-expression of the human malate–aspartate NADH shuttle member Aralar I in PK15 cells improves energy metabolism and enhances proliferation of porcine circovirus-2. RSC Adv 6:61268-77.|
Abstract: The considerable losses sustained by the pig industry due to porcine circovirus-2 (PCV2) could be avoided by using an attenuated vaccine. However, production of an attenuated PCV2 vaccine has not been satisfactory. Recently, we over-expressed the human malate–aspartate NADH shuttle member Aralar I in PK15 cells to enhance proliferation of PCV2. Compared with control PK15 cells, lactate accumulation in Aralar I over-expressing cells decreased by 44.8 % (p < 0.01), whereas the mitochondrial NADH and pyruvate concentrations increased by 50 % (p < 0.05) and 35.4 % (p < 0.05), respectively. The ATP/ADP ratio and O2 uptake rate also increased by 39% (p < 0.05) and 37.8 % (p < 0.01), respectively. Furthermore, the glutamine consumption rate increased by 36.4 % (p < 0.01). Finally, compared with the PCV2 yield of infected control PK15 cells, the PCV2 yield of infected Aralar I over-expression cells was enhanced by 42.5% (p < 0.05). These results revealed that over-expression of Aralar I can improve the energy metabolism of PK15 cells and also enhance the replication of the PCV2 virus in PK15 cells. This study presents a method to improve energy metabolism and provides a valuable thinking to enhance virus vaccine production.
Labels: MiParea: Respiration, Genetic knockout;overexpression, mt-Medicine, Pharmacology;toxicology Pathology: Infectious
Organism: Pig Tissue;cell: Kidney Preparation: Intact cells
Regulation: Ion;substrate transport
Labels, 2018-10, Virus, CN