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| [[File:O2k-TIP2k.jpg|right|300px|caption]] | | #REDIRECT [[MiPNet16.03 IOC65]] |
| '''O2k-Workshop on High-Resolution Respirometry: O2k-Basic and TPP-Basic'''
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| Schröcken, Vorarlberg, Austria 10 - 15 December, 2011.
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| Hotel Körbersee
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| More information on IOC62: http://www.oroboros.at/index.php?nextcourse
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| For some pictures, see [[IOC61_Photos|IOC61]]
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| '''Contact''': [[Has mailaddress::{{{mailaddress|katrin.stecher@oroboros.at}}}]]
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| *[http://wiki.oroboros.at/index.php/Special:AbstractsExtension?event=IOC62 Add your Abstract >>]
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| == Combined Workshop O2k-Basic and TPP-Basic with Parallel Sessions==
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| * Groups will be working in parallel sessions (O2k-Basic and TPP-Basic).
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| * The workshop programme is complemented by combined lectures ("General topics") and experiments run in "Special interest groups".
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| * Short presentations of 'Hot topics in Mitochondrial Physiology' extend the discussions on respiratory protocols.
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| [http://www.oroboros.at/index.php?id=nextcourse Please register] for '''O2k Basic''' or '''TPP Basic''' according to your specific needs and interest.
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| ===Preliminary Programme===
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| ::'''A: Introductory lectures and hands-on experiments for basic HRR in small working teams:'''
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| '''A1. General [[HRR]] topics'''
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| * O2k-assembly
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| * [[Polarographic oxygen sensor]] (OroboPOS) service
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| * POS calibration
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| * [[DatLab 4.3|DatLab]] software: principles and operation
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| * [[Titration-Injection-microPump]] - TIP2k.
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| '''A2. Hands-on experiments with the O2k'''
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| * Measurement of [[instrumental background oxygen flux]], manual and TIP2k-feedback control
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| * Intact cells: Respirometric reference assay with yeast, [[phosphorylation control protocol]]
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| * Preparation of [[permeabilized muscle fibre]]s
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| * HRR with permeabilized muscle fibres.
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| '''A3. Presentation and interpretation of [[oxygen flux]]'''
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| * Normalization of respiration per tissue or per [[mitochondrial marker]]
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| * Coupling control ratios
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| * Substrate control ratios
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| * Generalized flux control ratios.
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| ::'''B: Introductory lectures and hands-on experiments for basic TPP<sup>+</sup> (mitochondrial membrane potential) in small working teams'''
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| * Hands-on MultiSensor TPP<sup>+</sup> applications, see [[TPP special interest group during an International Oxygraph Course]]
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| * From TPP<sup>+</sup> to mitochondrial membrane potential: unspecific binding, partitioning, chemical side effects.
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| ::'''C: General topics'''
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| * Overview on the O2k and DatLab: High-resolution respirometry (HRR)
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| * Overview on O2k-MultiSensor applications
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| :* Mitochondrial membrane potential (TPP+ electrode)
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| :* Acidification (pH electrode), pH-stat feedback control
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| :* Nitric oxide (amperometric)
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| * Introduction to fundamental elements of mitochondrial respiratory physiology
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| * Protocols in HRR: Design and examples of experimental protocols with intact cells, permeabilized cells and tissues, isolated mitochondria.
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| * Quality control - towards [[quality assurance]]
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| ::'''D: Special interest groups'''
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| * Oxygen kinetics: experimental examples with TIP2k-feedback control (oxystat) and data analysis,
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| * ADP kinetics: experimental examples with TIP2-steady state control and data analysis,
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| * Advanced experimental protocols
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| ==Participants and areas of interest==
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| == Tutors ==
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| * [[Gnaiger E|'''Erich Gnaiger''']] (Innsbruck Medical University, AT; OROBOROS INSTRUMENTS, AT)
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| * [[Fasching M|'''Mario Fasching''']] (OROBOROS INSTRUMENTS, AT)
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| * [[Pesta D|'''Dominik Pesta''']] (Innsbruck Medical University, AT)
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| * [[Sumbalova Z|'''Zuzana Sumbalova''']] (OROBOROS INSTRUMENTS, AT)
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| * [[Wiethuechter A|'''Anita Wiethuechter''']] (OROBOROS INSTRUMENTS, AT)
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| [[Category:IOC62]]
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| [[Category:OroboroPedia]]
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| [[Category:Events]]
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