Scandurra 2010 Adv Exp Med Biol: Difference between revisions
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|tissues=Blood cells | |tissues=Blood cells | ||
|preparations=Intact cells, Isolated | |preparations=Intact cells, Isolated mitochondria | ||
|injuries=Hypoxia | |injuries=Hypoxia | ||
|topics=O2 | |topics=O2 |
Revision as of 11:00, 26 February 2015
Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662:7-25. |
ยป PMID: 20204766, Bioblast.pdf
Scandurra FM, Gnaiger E (2010) Adv Exp Med Biol
Abstract: When oxygen supply to tissues is limiting, mitochondrial respiration and ATP production are compromised. To assess the bioenergetic consequences under normoxia and hypoxia, quantitative evaluation of mitochondrial oxygen kinetics is required. Using high-resolution respirometry, the "apparent Km" for oxygen or p50 of respiration in 32D cells was determined at 0.05 +/- 0.01 kPa (0.4 mmHg, 0.5 ยตM, 0.25% air saturation). Close agreement with p50 of isolated mitochondria indicates that intracellular gradients are small in small cells at routine activity. At intracellular pO2 <2 kPa (15 mmHg, 10% air saturation) in various tissues under normoxia, respiration is limited by >2% with a p50 of 0.05 kPa. Over-estimation of p50 at 0.4 kPa (3 mmHg) would imply significant (>17%) oxygen limitation of respiration under intracellular normoxia. Based on a critical review, we conclude that p50 ranges from 0.01 to 0.10 kPa in mitochondria and small cells in the absence of inhibitors of cytochrome c oxidase, whereas experimental artefacts explain the controversial >200-fold range of p50 in the literature on mitochondrial oxygen kinetics.
โข O2k-Network Lab: AT_Innsbruck_Gnaiger E, AT Innsbruck OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Stress:Hypoxia Organism: Mouse Tissue;cell: Blood cells Preparation: Intact cells, Isolated mitochondria
Regulation: O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property. Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k