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| [[File:Tube and Syringe Racks with O2k.JPG|right|240px|Tube and Syringe Racks with O2k]]
| | #REDIRECT [[Oroboros O2k]] |
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| The [[Oroboros O2k]] is the modular system for [[high-resolution respirometry]] in mitochondrial physiology and pathology. The [[O2k-Fluorometer]] fully supports all [[O2k-MultiSensor| O2k-Multisensor Modules]].
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| __TOC__
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| == An Integrated Systems Approach: High-resolution respirometry ==
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| [[File:O2k-Core basic.jpg|right|400px]]
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| The Oroboros O2k is the unique state-of-the-art instrument for HRFR - where high-resolution counts:
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| * with small amounts of cells or tissue,
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| * at low respiratory activities,
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| * multiple substrate-uncoupler-inhibitor titrations (SUIT protocols) with resolution of changes in oxygen flux over prolonged incubation times,
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| * flexibility of O2k-MultiSensor applications,
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| * fast transitions at low oxygen levels,
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| * stability and quality control.
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| High-resolution respirometry is the result of our long-term expertise in instrumental design, software development, mitochondrial physiology, clinical and pharmacological applications, and [[O2k-Network| world-wide scientific cooperation]]. The innovative O2k-developments provide a system of highest quality and distinguish high-resolution respirometry as a method and concept.
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| Today, such high resolution meets rapidly increasing demands, reflected in more than [[O2k-Publications| 1,600 O2k-Publications]]. Applications of the O2k in leading [[O2k-Network| O2k-Network Labs]] are the driving forces for a rapid advancement of mitochondrial physiology. Open access to the [[O2k-Manual]] is an integral part of the concept of [[OROBOROS INSTRUMENTS]] on [[Gentle Science]] and Scientific Social Responsibility. With sharing of [[O2k-Protocols]] and implementation of quality control, the study of mitochondrial and cell respiration emerges as a quantitative science. Thus high-resolution respirometry provides the foundation for comparative mitochondrial physiology, biomedical research, translation into clinical applications, establishing quantitative functional mitochondrial diagnosis.
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| * Sole source information: [mailto:instruments@oroboros.at| instruments@oroboros.at]
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| * [[Oroboros FAQ|Frequently Asked Questions]]
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| The Oroboros O2k sets a world-wide standard in high-resolution respirometry - for mitochondrial studies in cell physiology and pathology. Developments progress [[beyond oxygen]].
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| In contrast to merely offering an oxygen measuring device, the Oroboros O2k is integrated into a systems approach to high-resolution respirometry. This spans from experimental tools which are invaluable in the lab (e.g. [[ISS]]), or little aids that are crucial for tissue preparation (forceps), mitochondrial respration medium ([[MiR05-Kit]]), [[O2k-Protocols| substrate-uncoupler-inhibitor titration protocols]], and the unique [[O2k-Workshops]].
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| == O2k - modular design ==
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| [[File:O2k Multisensor.jpg|right|400px]]
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| * [[O2k-Core]]: Substrate-uncoupler-inhibitor titrations (SUIT), OXPHOS flux control (L, P, E), manual titrations, core system for modular add-on extension to use the TIP2k and O2k-MultiSensor Modules.
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| * [[TIP2k-Module|Titration-Injection microPump TIP2k]]: The O2k-TIP2k combination allows operation with programmable titration regimes, feedback control, and at steady-states, yielding a new flexibility in experimental design by combining the technical advantages of closed and open systems approaches. Automatic titrations for kinetics; feedback-controlled instrumental background tests; respiration in a closed or open system, in aerobic-anaerobic transitions or at steady-state oxygen concentrations, covering the entire physiological oxygen range and the extremes of hypoxia.
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| * [[O2k-Catalogue: O2k-MultiSensor|O2k-MultiSensor Module]]: HRR combined with [[O2k-Fluo LED2-Module|fluorometry]] (ROS production, ATP production, mitochondrial membrane potential, Ca2+, pH), [[O2k-TPP+ ISE-Module|potentiometry]] (pH, TPP+ or TPMP+ for mitochondrial membrane potential, Ca2+) and [[O2k-NO Amp-Module|amperometry]] (NO and oxygen kinetics).
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| * Core-Accessories and Auxiliary HRR-Tools are optionally available sets for high-resolution respirometry: [[MiPNet19.14 SOP Hamilton microsyringes|Microsyringes]], [[PBI-Shredder]], [[Microbalance-Set|Microbalance]], and [[O2k-Catalogue: HRR-Dissection Tools|HRR-Dissection Tools]].
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| == Further information ==
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| » [[MiPNet06.01| O2k-Overview]]
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| » [[High-resolution respirometry| High-resolution respirometry (HRR)]]
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| » [[MiPNet14.10| Top 10 Reasons]] for OROBOROS INSTRUMENTS
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| » [[MiPNet06.05 Specifications| O2k-Specifications]]
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| » [[Purchase#Low_running_costs| Quality and economy]]: high-output, low running costs
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| » [[Oroboros O2k-Catalogue| A guided tour to the Oxygraph-2k]]
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| » [[O2k-Fluorometer]]
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