Physiological temperature: Difference between revisions
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== Which temperature should be applied to determine mitochondrial function? == | == Which temperature should be applied to determine mitochondrial function? == | ||
We recommend determining mitochondrial respiratory activity at the physiologic temperature of your biologic system, no matter whether you work with [[MiPNet14.14 PermeabilizedFibrePreparation|biopsies]], [[MiPNet08.09 CellRespiration cultivated | We recommend determining mitochondrial respiratory activity at the physiologic temperature of your biologic system, no matter whether you work with [[MiPNet14.14 PermeabilizedFibrePreparation|biopsies]], [[MiPNet08.09 CellRespiration|cultivated cells]] or [[MiPNet12.11 MitoRespiration|isolated mitochondria]]. When measuring in an appropriate [[List of media for respirometry|medium]] (protecting and stabilizing mitochondrial function) stability is preserved even in isolated mitochondria. | ||
Revision as of 11:25, 9 August 2011
Which temperature should be applied to determine mitochondrial function?
We recommend determining mitochondrial respiratory activity at the physiologic temperature of your biologic system, no matter whether you work with biopsies, cultivated cells or isolated mitochondria. When measuring in an appropriate medium (protecting and stabilizing mitochondrial function) stability is preserved even in isolated mitochondria.