Adherent cells
From Bioblast
As is obvious from the FAQs respirometry with adherent cells is of major interest. Unfortunately, it is also true that adherent cells are a major problem for the rapid stirring required for high-resolution respirometry. Various work around have been tried, e.g.
- big beads in a wire cage
- glass coverslip of adherent cells on top of the stirrer, see Jones 2009 ExpNeurol
However, these methods are always a compromise. Experiences with these or other methods would be extremely welcome on this page and on its accompanying Discussion page!
