Talk:MiPNet17.05 O2k-Fluo LED2-Module
From Bioblast
Titration and settings
- 10 Β΅l AmR - 5 Β΅M final conc. in chamber
- 4Β΅l HRP (500U/ml) - 1 U/ml final conc. in chamber* light intensity of LEDs: 1 mA ( DatLab: Amp polaristion voltage 100 mV) / Gain 1000
- H2O2 calibration with a 15.8 Β΅M H2O2 solution - 2012-06-20
- commercial stock: 880 mM
- dilution 1 (1:166.67): 150 Β΅l in 25 ml H2O = 5.28 mM
- dilution 2 (1:333.33): 150 Β΅l in 50 ml H2O + 500 Β΅l 1 mM HCl = 15.8 Β΅M
- β calibration: 2 * 5 Β΅l in 2 ml chamber = 2* 0.0395 Β΅M = total 0.079 Β΅M (= 79 nM)
- new H2O2 calibration with a 28.16 Β΅M H2O2 solution, valid from from 2012-06-22:
- commercial stock: 880 mM
- dilution 1 (1:125): 200 Β΅l in 25 ml H2O = 7.04 mM
- dilution 2 (1:250): 200 Β΅l in 50 ml H2O + 500 Β΅l 1 mM HCl = 28.16 Β΅M
- β calibration: 2 * 5 Β΅l in 2 ml chamber = 2* 0.0704 Β΅M = total 0.1408 Β΅M (= 140.8 nM)