Difference between revisions of "2-Deoxyglucose"
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|description='''2-Deoxyglucose''', also known as 2-deoxy-D-glucose is a glucose derivative which has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase . | |description='''2-Deoxyglucose''', also known as 2-deoxy-D-glucose is a glucose derivative which has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase . | ||
|info=[[Proton_flux]], [[O2k-pH_ISE-Module]] | |info=[[Proton_flux]], [[O2k-pH_ISE-Module]] | ||
Revision as of 10:40, 21 February 2020
- high-resolution terminology - matching measurements at high-resolution
2-Deoxyglucose
Description
2-Deoxyglucose, also known as 2-deoxy-D-glucose is a glucose derivative which has the 2-hydroxyl group replaced by hydrogen. It competitively inhibits glycolysis by blocking hexokinase and phosphohexoseisomerase .
Abbreviation: 2-DG
Reference: Proton_flux, O2k-pH_ISE-Module
MitoPedia topics: Inhibitor
Application in HRR
2-DG: 2-deoxy-D-glucose C6H1205); Sigma D6134, 1 g or 5 g, store at 2-8 °C; FW = 164.16
Preparation of 2 M stock solution (dissolved in H2O):
- 1) weigh 1g of 2-deoxy-D-glucose
- 2) dissolve 3.0458 mL H2O in a 10 mL-glass beaker
Oxygraph-2k Titration:
- Titration volume: 25 µL using a 50 µL syringe
- Final conc. in 2 mL O2k-chamber: 25 mM
