Difference between revisions of "Amytal"
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{{MitoPedia methods|type=Inhibitor ETS | {{MitoPedia methods|type=Inhibitor ETS | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Amy: Amytal''' (Amytal sodium salt, C<sub>11</sub>H<sub>17</sub>N<sub>2</sub>O<sub>3</sub>Na); Sigma A 4430, 50 g; FW = 248.25. ''The product is withdrawn from Sigma but some residual amounts are still available.'' | :::'''Amy: Amytal''' (Amytal sodium salt, C<sub>11</sub>H<sub>17</sub>N<sub>2</sub>O<sub>3</sub>Na); Sigma A 4430, 50 g; FW = 248.25. ''The product is withdrawn from Sigma but some residual amounts are still available.'' | ||
<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span> | ::::<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span> | ||
::::'''Preparation of 200 mM stock solution''' (dissolved in 99.9% ethanol): | |||
::::# Weigh 49.65 mg of amytal. | |||
::::# Dissolve in 10 ml 50% ethanol. | |||
:: | ::::# Divide into 500 µl aliquots (use dark glass vials - light sensitive). | ||
:: | ::::# Store at -20 °C. | ||
:: | {{MitoPedia topics | ||
:: | |mitopedia topic=Inhibitor | ||
|type=Inhibitor ETS | |||
}} | |||
Revision as of 09:58, 11 September 2020
- high-resolution terminology - matching measurements at high-resolution
Amytal
Description
Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is a barbiturate drug and an inhibitor of Complex I.
Abbreviation: Amy
Application in HRR
- Amy: Amytal (Amytal sodium salt, C11H17N2O3Na); Sigma A 4430, 50 g; FW = 248.25. The product is withdrawn from Sigma but some residual amounts are still available.
- Caution: Light sensitive and toxic!
- Preparation of 200 mM stock solution (dissolved in 99.9% ethanol):
- Weigh 49.65 mg of amytal.
- Dissolve in 10 ml 50% ethanol.
- Divide into 500 µl aliquots (use dark glass vials - light sensitive).
- Store at -20 °C.
MitoPedia topics: Inhibitor
