Difference between revisions of "Amytal"
From Bioblast
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|type=Inhibitor ETS | |type=Inhibitor ETS | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
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::::# Weigh 49.65 mg of amytal. | ::::# Weigh 49.65 mg of amytal. | ||
::::# Dissolve in 10 | ::::# Dissolve in 10 mL 50% ethanol. | ||
::::# Divide into 500 | ::::# Divide into 500 µL aliquots (use dark glass vials - light sensitive). | ||
::::# Store at -20 °C. | ::::# Store at -20 °C. | ||
{{MitoPedia methods|type=Inhibitor ETS | |||
}} | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Inhibitor | |mitopedia topic=Inhibitor | ||
|type=Inhibitor ETS | |type=Inhibitor ETS | ||
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Revision as of 09:59, 11 September 2020
- high-resolution terminology - matching measurements at high-resolution
Amytal
Description
Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is a barbiturate drug and an inhibitor of Complex I.
Abbreviation: Amy
Application in HRR
- Amy: Amytal (Amytal sodium salt, C11H17N2O3Na); Sigma A 4430, 50 g; FW = 248.25. The product is withdrawn from Sigma but some residual amounts are still available.
- Caution: Light sensitive and toxic!
- Preparation of 200 mM stock solution (dissolved in 99.9% ethanol):
- Weigh 49.65 mg of amytal.
- Dissolve in 10 mL 50% ethanol.
- Divide into 500 µL aliquots (use dark glass vials - light sensitive).
- Store at -20 °C.
MitoPedia topics:
Inhibitor
