Difference between revisions of "Amytal"
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''Amy: Amytal''' (Amytal sodium salt, C<sub>11</sub>H<sub>17</sub>N<sub>2</sub>O<sub>3</sub>Na); Sigma A 4430, 50 g; FW = 248.25 | '''Amy: Amytal''' (Amytal sodium salt, C<sub>11</sub>H<sub>17</sub>N<sub>2</sub>O<sub>3</sub>Na); Sigma A 4430, 50 g; FW = 248.25. ''The product is withdrawn from Sigma but some residual amounts are still available.'' | ||
<span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span> | <span style="color:#8B008B"> '''Caution:''' Light sensitive and toxic!</span> | ||
'''Preparation of 200 mM stock solution''' (dissolved in 99.9% | '''Preparation of 200 mM stock solution''' (dissolved in 99.9% ethanol): | ||
::1) | ::1) Weigh 49.65 mg of amytal. | ||
::2) | ::2) Dissolve in 10 ml 50% ethanol. | ||
::3) | ::3) Divide into 500 µl aliquots (use dark glass vials - light sensitive). | ||
::4) | ::4) Store at -20 °C. | ||
Revision as of 15:49, 2 May 2014
- high-resolution terminology - matching measurements at high-resolution
Amytal
Description
Amytal sodium salt (synonym: amobarbital; 5-Ethyl-5-isoamylbarbituric acid) is a barbiturate drug and an inhibitor of Complex I.
Abbreviation: Amy
MitoPedia topics: Inhibitor
Application in HRR
Amy: Amytal (Amytal sodium salt, C11H17N2O3Na); Sigma A 4430, 50 g; FW = 248.25. The product is withdrawn from Sigma but some residual amounts are still available.
Caution: Light sensitive and toxic!
Preparation of 200 mM stock solution (dissolved in 99.9% ethanol):
- 1) Weigh 49.65 mg of amytal.
- 2) Dissolve in 10 ml 50% ethanol.
- 3) Divide into 500 µl aliquots (use dark glass vials - light sensitive).
- 4) Store at -20 °C.
