Difference between revisions of "Baris 2015 Am J Physiol Regul Integr Comp Physiol"
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|title=Baris TZ, Crawford DL, Oleksiak MF (2015) Acclimation and acute temperature effects on population differences in oxidative phosphorylation. Am J Physiol Regul Integr Comp Physiol [Epub ahead of print]. | |title=Baris TZ, Crawford DL, Oleksiak MF (2015) Acclimation and acute temperature effects on population differences in oxidative phosphorylation. Am J Physiol Regul Integr Comp Physiol [Epub ahead of print]. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/26582639 PMID: 26582639] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/26582639 PMID: 26582639] | ||
|authors=Baris TZ, Crawford DL, Oleksiak MF | |authors=Baris TZ, Crawford DL, Oleksiak MF | ||
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|abstract=Temperature changes affect metabolism on acute, acclimatory and evolutionary time scales. To better understand temperature's affect on metabolism at these different time scales, we quantified cardiac oxidative phosphorylation (OxPhos) in three ''Fundulus'' taxa acclimated to 12°C and 28°C and measured at three acute temperatures (12°C, 20°C, and 28°C). The ''Fundulus'' taxa (northern Maine and southern Georgia ''F. heteroclitus'', and a sister taxa, ''F. grandis'') were used to identify evolved changes in OxPhos. Cardiac OxPhos metabolism was quantified by measuring six traits: State 3 (ADP and substrate dependent mitochondrial respiration), E State (uncoupled mitochondrial activity), Complex I, II, and IV activities, and LEAK ratio. Acute temperature affected all OxPhos traits. Acclimation only significantly affected State 3 and LEAK ratio. Populations were significantly different for State 3. In addition to direct effects, there were significant interactions between acclimation and population for Complex I and between population and acute temperature for State 3. Further analyses suggest that acclimation alters the acute temperature response for State 3, E State, and Complexes I and II: at the low acclimation temperature, the acute response was dampened at low assay temperatures, and at the high acclimation temperature, the acute response was dampened at high assay temperatures. Closer examination of the data also suggests that differences in State 3 respiration and Complex I activity between populations were greatest between fish acclimated to low temperatures when assayed at high temperatures, suggesting that differences between the populations become more apparent at the edges of their thermal range. | |abstract=Temperature changes affect metabolism on acute, acclimatory and evolutionary time scales. To better understand temperature's affect on metabolism at these different time scales, we quantified cardiac oxidative phosphorylation (OxPhos) in three ''Fundulus'' taxa acclimated to 12°C and 28°C and measured at three acute temperatures (12°C, 20°C, and 28°C). The ''Fundulus'' taxa (northern Maine and southern Georgia ''F. heteroclitus'', and a sister taxa, ''F. grandis'') were used to identify evolved changes in OxPhos. Cardiac OxPhos metabolism was quantified by measuring six traits: State 3 (ADP and substrate dependent mitochondrial respiration), E State (uncoupled mitochondrial activity), Complex I, II, and IV activities, and LEAK ratio. Acute temperature affected all OxPhos traits. Acclimation only significantly affected State 3 and LEAK ratio. Populations were significantly different for State 3. In addition to direct effects, there were significant interactions between acclimation and population for Complex I and between population and acute temperature for State 3. Further analyses suggest that acclimation alters the acute temperature response for State 3, E State, and Complexes I and II: at the low acclimation temperature, the acute response was dampened at low assay temperatures, and at the high acclimation temperature, the acute response was dampened at high assay temperatures. Closer examination of the data also suggests that differences in State 3 respiration and Complex I activity between populations were greatest between fish acclimated to low temperatures when assayed at high temperatures, suggesting that differences between the populations become more apparent at the edges of their thermal range. | ||
|keywords=Fundulus, State 3, Enzyme complexes, Mitochondria | |keywords=Fundulus, State 3, Enzyme complexes, Mitochondria | ||
|mipnetlab=US FL Miami Crawford DL | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
Revision as of 17:44, 23 December 2015
| Baris TZ, Crawford DL, Oleksiak MF (2015) Acclimation and acute temperature effects on population differences in oxidative phosphorylation. Am J Physiol Regul Integr Comp Physiol [Epub ahead of print]. |
Baris TZ, Crawford DL, Oleksiak MF (2015) Am J Physiol Regul Integr Comp Physiol
Abstract: Temperature changes affect metabolism on acute, acclimatory and evolutionary time scales. To better understand temperature's affect on metabolism at these different time scales, we quantified cardiac oxidative phosphorylation (OxPhos) in three Fundulus taxa acclimated to 12°C and 28°C and measured at three acute temperatures (12°C, 20°C, and 28°C). The Fundulus taxa (northern Maine and southern Georgia F. heteroclitus, and a sister taxa, F. grandis) were used to identify evolved changes in OxPhos. Cardiac OxPhos metabolism was quantified by measuring six traits: State 3 (ADP and substrate dependent mitochondrial respiration), E State (uncoupled mitochondrial activity), Complex I, II, and IV activities, and LEAK ratio. Acute temperature affected all OxPhos traits. Acclimation only significantly affected State 3 and LEAK ratio. Populations were significantly different for State 3. In addition to direct effects, there were significant interactions between acclimation and population for Complex I and between population and acute temperature for State 3. Further analyses suggest that acclimation alters the acute temperature response for State 3, E State, and Complexes I and II: at the low acclimation temperature, the acute response was dampened at low assay temperatures, and at the high acclimation temperature, the acute response was dampened at high assay temperatures. Closer examination of the data also suggests that differences in State 3 respiration and Complex I activity between populations were greatest between fish acclimated to low temperatures when assayed at high temperatures, suggesting that differences between the populations become more apparent at the edges of their thermal range. • Keywords: Fundulus, State 3, Enzyme complexes, Mitochondria
• O2k-Network Lab: US FL Miami Crawford DL
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Stress:Temperature
Tissue;cell: Heart
Coupling state: LEAK, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
HRR: Oxygraph-2k
Labels, [Epub ahead of print], 2012-12
