Bodart-Santos 2019 Stem Cell Res Ther: Difference between revisions
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Latest revision as of 16:29, 4 February 2020
| Bodart-Santos V, de Carvalho LRP, de Godoy MA, Batista AF, Saraiva LM, Lima LG, Abreu CA, De Felice FG, Galina A, Mendez-Otero R, Ferreira ST (2019) Extracellular vesicles derived from human Wharton's jelly mesenchymal stem cells protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-ฮฒ oligomers. Stem Cell Res Ther 10:332. |
Bodart-Santos V, de Carvalho LRP, de Godoy MA, Batista AF, Saraiva LM, Lima LG, Abreu CA, De Felice FG, Galina A, Mendez-Otero R, Ferreira ST (2019) Stem Cell Res Ther
Abstract: Mesenchymal stem cells (MSCs) have been explored as promising tools for treatment of several neurological and neurodegenerative diseases. MSCs release abundant extracellular vesicles (EVs) containing a variety of biomolecules, including mRNAs, miRNAs, and proteins. We hypothesized that EVs derived from human Wharton's jelly would act as mediators of the communication between hMSCs and neurons and could protect hippocampal neurons from damage induced by Alzheimer's disease-linked amyloid beta oligomers (AฮฒOs).
We isolated and characterized EVs released by human Wharton's jelly mesenchymal stem cells (hMSC-EVs). The neuroprotective action of hMSC-EVs was investigated in primary hippocampal cultures exposed to AฮฒOs.
hMSC-EVs were internalized by hippocampal cells in culture, and this was enhanced in the presence of AฮฒOs in the medium. hMSC-EVs protected hippocampal neurons from oxidative stress and synapse damage induced by AฮฒOs. Neuroprotection by hMSC-EVs was mediated by catalase and was abolished in the presence of the catalase inhibitor, aminotriazole.
hMSC-EVs protected hippocampal neurons from damage induced by AฮฒOs, and this was related to the transfer of enzymatically active catalase contained in EVs. Results suggest that hMSC-EVs should be further explored as a cell-free therapeutic approach to prevent neuronal damage in Alzheimer's disease.
โข Bioblast editor: Plangger M โข O2k-Network Lab: BR Rio de Janeiro Galina A, BR Rio de Janeiro Institute Biomedical Chemistry
Labels: MiParea: Respiration
Pathology: Alzheimer's
Organism: Human Tissue;cell: Stem cells
HRR: Oxygraph-2k
Labels, 2020-02
