Difference between revisions of "Boushel 2013 Abstract MiP2013"
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|event=MiP2013 | |event=MiP2013 | ||
|abstract=Maximal oxygen consumption (''V''<sub>O2</sub>) in the organism is defined by the integrated functional capacities of multiple organ systems and characterized by proportionate design of the structural components of the O2 cascade from lung to mitochondria across a wide range of species. Yet, humans exhibit an excess capacity of muscle mitochondrial OXPHOS capacity relative to convective O2 delivery. This pattern holds with aging and in chronic diseases such as chronic obstructive lung disease and type 2 diabetes despite a lower expression and/or dysfunction of mitochondria. Muscle diffusional O<sub>2</sub> conductance (''D''<sub>O2</sub>) is largely dependent on the capillary volume:muscle interface which influences the mean transit time of erythrocytes and off-loading of O2 from haemoglobin at a given ''p''50 and blood flow. Convective O<sub>2</sub> delivery remains a dominant factor defining maximal ''V''<sub>O2</sub> as revealed by comparison of muscle ''D''<sub>O2</sub>, mean capillary ''p''<sub>O2</sub>, and ''V''<sub>O2</sub> during exercise engaging small to large muscle groups. The increase in muscle VO2 with endurance training in young, healthy humans is characterized by proportional increases in O<sub>2</sub> convection and diffusion, with variable enhancement of mitochondrial OXPHOS capacity, which remains in excess to maximize the ''p''<sub>O2</sub> gradient from the red blood cell to cytochrome ''c'' oxidase. | |abstract=Maximal oxygen consumption (''V''<sub>O2</sub>) in the organism is defined by the integrated functional capacities of multiple organ systems and characterized by proportionate design of the structural components of the O2 cascade from lung to mitochondria across a wide range of species. Yet, humans exhibit an excess capacity of muscle mitochondrial OXPHOS capacity relative to convective O2 delivery. This pattern holds with aging and in chronic diseases such as chronic obstructive lung disease and type 2 diabetes despite a lower expression and/or dysfunction of mitochondria. Muscle diffusional O<sub>2</sub> conductance (''D''<sub>O2</sub>) is largely dependent on the capillary volume:muscle interface which influences the mean transit time of erythrocytes and off-loading of O2 from haemoglobin at a given ''p''50 and blood flow. Convective O<sub>2</sub> delivery remains a dominant factor defining maximal ''V''<sub>O2</sub> as revealed by comparison of muscle ''D''<sub>O2</sub>, mean capillary ''p''<sub>O2</sub>, and ''V''<sub>O2</sub> during exercise engaging small to large muscle groups. The increase in muscle VO2 with endurance training in young, healthy humans is characterized by proportional increases in O<sub>2</sub> convection and diffusion, with variable enhancement of mitochondrial OXPHOS capacity, which remains in excess to maximize the ''p''<sub>O2</sub> gradient from the red blood cell to cytochrome ''c'' oxidase. | ||
|keywords=Exercise | |||
|mipnetlab=DK Copenhagen Boushel RC | |mipnetlab=DK Copenhagen Boushel RC | ||
}} | }} | ||
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|area=Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style | |area=Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style | ||
|organism=Human | |organism=Human | ||
|tissues=Skeletal muscle | |||
|preparations=Intact Organism, Permeabilized cells, Permeabilized tissue | |||
|diseases=Aging; senescence, COPD, Diabetes, Other | |||
|couplingstates=OXPHOS | |couplingstates=OXPHOS | ||
|substratestates=CI, CII, CI+II | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional= | |additional=MiP2013, Prep | ||
}} | }} | ||
__TOC__ | __TOC__ | ||
Revision as of 14:50, 13 August 2013
| Boushel RC (2013) Convective O2 delivery, diffusion and mitochondrial OXPHOS components of VO2 during exercise in health and disease. Mitochondr Physiol Network 18.08. |
Link:
Boushel RC (2013)
Event: MiP2013
Maximal oxygen consumption (VO2) in the organism is defined by the integrated functional capacities of multiple organ systems and characterized by proportionate design of the structural components of the O2 cascade from lung to mitochondria across a wide range of species. Yet, humans exhibit an excess capacity of muscle mitochondrial OXPHOS capacity relative to convective O2 delivery. This pattern holds with aging and in chronic diseases such as chronic obstructive lung disease and type 2 diabetes despite a lower expression and/or dysfunction of mitochondria. Muscle diffusional O2 conductance (DO2) is largely dependent on the capillary volume:muscle interface which influences the mean transit time of erythrocytes and off-loading of O2 from haemoglobin at a given p50 and blood flow. Convective O2 delivery remains a dominant factor defining maximal VO2 as revealed by comparison of muscle DO2, mean capillary pO2, and VO2 during exercise engaging small to large muscle groups. The increase in muscle VO2 with endurance training in young, healthy humans is characterized by proportional increases in O2 convection and diffusion, with variable enhancement of mitochondrial OXPHOS capacity, which remains in excess to maximize the pO2 gradient from the red blood cell to cytochrome c oxidase.
• Keywords: Exercise
• O2k-Network Lab: DK Copenhagen Boushel RC
Labels: MiParea: Respiration, mt-Biogenesis;mt-density, Exercise physiology;nutrition;life style Pathology: Aging; senescence"Aging; senescence" is not in the list (Aging;senescence, Alzheimer's, Autism, Cancer, Cardiovascular, COPD, Diabetes, Inherited, Infectious, Myopathy, ...) of allowed values for the "Diseases" property., COPD, Diabetes, Other
Organism: Human Tissue;cell: Skeletal muscle Preparation: Intact Organism"Intact Organism" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., Permeabilized cells, Permeabilized tissue
Coupling state: OXPHOS
HRR: Oxygraph-2k
MiP2013, Prep
Affiliations and author contributions
1 - Heart and Circulatory Section, Dept of Biomedical Sciences, University of Copenhagen; Department of Anaesthesia, Bispebjerg Hospital, Denmark.
Email: boushel@sund.ku.dk
