Difference between revisions of "Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"
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#E_S+Rot | #E_S+Rot | ||
#ROX_AntimycinA | #ROX_AntimycinA | ||
|mipnetlab=US CA San Diego Cannon DT | |||
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{{Labeling | {{Labeling | ||
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|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|couplingstates=LEAK, OXPHOS, | |couplingstates=LEAK, OXPHOS, ET | ||
|pathways=F, N, S, NS, ROX | |pathways=F, N, S, NS, ROX | ||
|additional=Palmitoylcarnitine | |additional=Palmitoylcarnitine | ||
}} | }} | ||
Latest revision as of 13:03, 18 March 2018
| Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Cannon DT (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
- Saponin permeabilized skeletal muscle fiber bundles
- Protocol:
- 0.5mM Malate
- 50 µM palmitoyl-CoA + 2mM carnitine
- 5mM ADP
- From here on out, various combinations for titration protocol
- Coupling states:
- LEAK_M+Palmitoylcarnitine
- P_M+Palmitoylcarnitine
- P_M+Palmitoylcarnitine+S
- E_O+CCCP titrations
- E_S+Rot
- ROX_AntimycinA
• O2k-Network Lab: US CA San Diego Cannon DT
Labels:
Organism: Human, Mouse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET
Pathway: F, N, S, NS, ROX
Palmitoylcarnitine
