Difference between revisions of "Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"

Latest revision as of 13:03, 18 March 2018

Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum

Link: O2k-Network Discussion Forum

Cannon DT (2015)

Event: Fatty Acid Oxidation O2k-Network Discussion Forum

Saponin permeabilized skeletal muscle fiber bundles

Protocol:

0.5mM Malate
50 µM palmitoyl-CoA + 2mM carnitine
5mM ADP
From here on out, various combinations for titration protocol

Coupling states:

LEAK_M+Palmitoylcarnitine
P_M+Palmitoylcarnitine
P_M+Palmitoylcarnitine+S
E_O+CCCP titrations
E_S+Rot
ROX_AntimycinA

• O2k-Network Lab: US CA San Diego Cannon DT

Labels:

Organism: Human, Mouse Tissue;cell: Skeletal muscle Preparation: Permeabilized tissue

Coupling state: LEAK, OXPHOS, ET Pathway: F, N, S, NS, ROX

Palmitoylcarnitine

@@ Line 5: / Line 5: @@
 |year=2015
 |event=Fatty Acid Oxidation O2k-Network Discussion Forum
-|abstract=Following your email from yesterday, I am sending you a graph with some mitochondrial respiration measurements obtained from the left ventricular human tissue of patients undergoing CABG surgery. As can be seen from the graph, we used FFA substrate to evoke the State 2 and State 3 respiration, and then followed with other substrates and an uncoupler. FFA substrate was palmitoyl-carnitine (40 ?M concentration). We performed quite a few of experiments using palmitoyl-carnitine, also in the range of concentrations from 1 – 40 ?M. We usually see a gradual increase in State 3 respiration with concentration increments (1, 2.5, 5, 10, 15, 40 ?M). However, the respiration is always lower then when pyruvate is used (although in much higher concentration, from 2 mM up). All of the observations come from the human left ventricular tissue, that we almost exclusively use in past couple of years for our experiments on mitochondria (we also measured activity of some enzymes and protein expression).
+|abstract=*Saponin permeabilized skeletal muscle fiber bundles
+*'''Protocol''':
+#0.5mM Malate
+#50 µM palmitoyl-CoA + 2mM carnitine
+#5mM ADP
+#From here on out, various combinations for titration protocol
+*Coupling states:
+#LEAK_M+Palmitoylcarnitine
+#P_M+Palmitoylcarnitine
+#P_M+Palmitoylcarnitine+S
+#E_O+CCCP titrations
+#E_S+Rot
+#ROX_AntimycinA
+|mipnetlab=US CA San Diego Cannon DT
 }}
 {{Labeling
-|substratestates=FAO
+|organism=Human, Mouse
-|additional=Palmitoylcarnitine
+|tissues=Skeletal muscle
+|preparations=Permeabilized tissue
+|couplingstates=LEAK, OXPHOS, ET
+|pathways=F, N, S, NS, ROX
+|additional=Palmitoylcarnitine
 }}