Difference between revisions of "Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"
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|year=2015 | |year=2015 | ||
|event=Fatty Acid Oxidation O2k-Network Discussion Forum | |event=Fatty Acid Oxidation O2k-Network Discussion Forum | ||
|abstract=*Saponin permeabilized skeletal muscle fiber bundles | |||
*'''Protocol''': | |||
#0.5mM Malate | |||
#50 µM palmitoyl-CoA + 2mM carnitine | |||
#5mM ADP | |||
#From here on out, various combinations for titration protocol | |||
*Coupling states: | |||
#LEAK_M+Palmitoylcarnitine | |||
#P_M+Palmitoylcarnitine | |||
#P_M+Palmitoylcarnitine+S | |||
#E_O+CCCP titrations | |||
#E_S+Rot | |||
#ROX_AntimycinA | |||
|mipnetlab=US CA San Diego Cannon DT | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
| | |organism=Human, Mouse | ||
|additional= | |tissues=Skeletal muscle | ||
|preparations=Permeabilized tissue | |||
|couplingstates=LEAK, OXPHOS, ET | |||
|pathways=F, N, S, NS, ROX | |||
|additional=Palmitoylcarnitine | |||
}} | }} | ||
Latest revision as of 13:03, 18 March 2018
| Cannon 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Cannon DT (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
- Saponin permeabilized skeletal muscle fiber bundles
- Protocol:
- 0.5mM Malate
- 50 µM palmitoyl-CoA + 2mM carnitine
- 5mM ADP
- From here on out, various combinations for titration protocol
- Coupling states:
- LEAK_M+Palmitoylcarnitine
- P_M+Palmitoylcarnitine
- P_M+Palmitoylcarnitine+S
- E_O+CCCP titrations
- E_S+Rot
- ROX_AntimycinA
• O2k-Network Lab: US CA San Diego Cannon DT
Labels:
Organism: Human, Mouse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET
Pathway: F, N, S, NS, ROX
Palmitoylcarnitine
