Difference between revisions of "Cardinale 2018 Front Physiol"

Latest revision as of 17:07, 7 March 2020

Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R (2018) Superior intrinsic mitochondrial respiration in women than in men. Front Physiol 9:1133.

» PMID: 30174617 Open Access

Cardinale Daniele A, Larsen Filip J, Schiffer Tomas A, Morales-Alamo David, Ekblom Bjoern, Calbet Jose AL, Holmberg Hans-Christer, Boushel Robert C (2018) Front Physiol

Abstract: Sexual dimorphism is apparent in humans, however, to date no studies have investigated mitochondrial function focusing on intrinsic mitochondrial respiration (i.e., mitochondrial respiration for a given amount of mitochondrial protein) and mitochondrial oxygen affinity (p_50mito) in relation to biological sex in human. A skeletal muscle biopsy was donated by nine active women, and ten men matched for maximal oxygen consumption (V_O₂max) and by nine endurance trained men. Intrinsic mitochondrial respiration, assessed in isolated mitochondria, was higher in women compared to men when activating Complex I (CI_P) and Complex I+II (CI+II_P) (p < 0.05), and was similar to trained men (CI_P, p = 0.053; CI+II_P, p = 0.066). Proton leak and p_50mito were higher in women compared to men independent of V_O₂max. In conclusion, significant novel differences in mitochondrial oxidative function, intrinsic mitochondrial respiration and p_50mito exist between women and men. These findings may represent an adaptation in the oxygen cascade in women to optimize muscle oxygen uptake to compensate for a lower oxygen delivery during exercise. • Keywords: OXPHOS, Endurance performance, Mitochondria, Mitochondrial function, Sexual dimorphism, Skeletal muscle • Bioblast editor: Plangger M • O2k-Network Lab: SE Stockholm Larsen FJ, SE Uppsala Liss P, SE Stockholm Boushel RC, SE Stockholm Cardinale DA

Labels: MiParea: Respiration, Gender, Exercise physiology;nutrition;life style

Organism: Human Tissue;cell: Skeletal muscle Preparation: Isolated mitochondria

Regulation: Oxygen kinetics Coupling state: OXPHOS Pathway: F, N, NS HRR: Oxygraph-2k

Labels, 2018-09, VO2max, Alert2020

@@ Line 2: / Line 2: @@
 |title=Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R (2018) Superior intrinsic mitochondrial respiration in women than in men. Front Physiol 9:1133.
 |info=[https://www.ncbi.nlm.nih.gov/pubmed/30174617  PMID: 30174617  Open Access]
-|authors=Cardinale DA, Larsen FJ, Schiffer TA, Morales-Alamo D, Ekblom B, Calbet JAL, Holmberg HC, Boushel R
+|authors=Cardinale Daniele A, Larsen Filip J, Schiffer Tomas A, Morales-Alamo David, Ekblom Bjoern, Calbet Jose AL, Holmberg Hans-Christer, Boushel Robert C
 |year=2018
 |journal=Front Physiol
-|abstract=Sexual dimorphism is apparent in humans, however, to date no studies have investigated mitochondrial function focusing on intrinsic mitochondrial respiration (i.e., mitochondrial respiration for a given amount of mitochondrial protein) and mitochondrial oxygen affinity (p50<sub>mito</sub>) in relation to biological sex in human. A skeletal muscle biopsy was donated by nine active women, and ten men matched for maximal oxygen consumption (VO<sub>2max</sub>) and by nine endurance trained men. Intrinsic mitochondrial respiration, assessed in isolated mitochondria, was higher in women compared to men when activating complex I (CI<sub>P</sub>) and complex I+II (CI+II<sub>P</sub>) (p < 0.05), and was similar to trained men (CI<sub>P</sub>, p = 0.053; CI+II<sub>P</sub>, p = 0.066). Proton leak and p50<sub>mito</sub> were higher in women compared to men independent of VO<sub>2max</sub>. In conclusion, significant novel differences in mitochondrial oxidative function, intrinsic mitochondrial respiration and p50<sub>mito</sub> exist between women and men. These findings may represent an adaptation in the oxygen cascade in women to optimize muscle oxygen uptake to compensate for a lower oxygen delivery during exercise.
+|abstract=Sexual dimorphism is apparent in humans, however, to date no studies have investigated mitochondrial function focusing on intrinsic mitochondrial respiration (i.e., mitochondrial respiration for a given amount of mitochondrial protein) and mitochondrial oxygen affinity (''p''<sub>50mito</sub>) in relation to biological sex in human. A skeletal muscle biopsy was donated by nine active women, and ten men matched for maximal oxygen consumption (''V''<sub>O<sub>2</sub>max</sub>) and by nine endurance trained men. Intrinsic mitochondrial respiration, assessed in isolated mitochondria, was higher in women compared to men when activating Complex I (CI<sub>P</sub>) and Complex I+II (CI+II<sub>P</sub>) (''p'' < 0.05), and was similar to trained men (CI<sub>P</sub>, ''p'' = 0.053; CI+II<sub>P</sub>, ''p'' = 0.066). Proton leak and ''p''<sub>50mito</sub> were higher in women compared to men independent of ''V''<sub>O<sub>2</sub>max</sub>. In conclusion, significant novel differences in mitochondrial oxidative function, intrinsic mitochondrial respiration and ''p''<sub>50mito</sub> exist between women and men. These findings may represent an adaptation in the oxygen cascade in women to optimize muscle oxygen uptake to compensate for a lower oxygen delivery during exercise.
 |keywords=OXPHOS, Endurance performance, Mitochondria, Mitochondrial function, Sexual dimorphism, Skeletal muscle
 |editor=[[Plangger M]],
-|mipnetlab=SE Stockholm Larsen FJ, SE Uppsala Liss P, SE Stockholm Boushel RC
+|mipnetlab=SE Stockholm Larsen FJ, SE Uppsala Liss P, SE Stockholm Boushel RC, SE Stockholm Cardinale DA
 }}
 {{Labeling
@@ Line 15: / Line 15: @@
 |tissues=Skeletal muscle
 |preparations=Isolated mitochondria
+|topics=Oxygen kinetics
 |couplingstates=OXPHOS
 |pathways=F, N, NS
 |instruments=Oxygraph-2k
-|additional=Labels, 2018-09,
+|additional=Labels, 2018-09, VO2max, Alert2020,
 }}