Difference between revisions of "Catalase"
From Bioblast
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::1) Use 'units/mg solid' for your calculations | ::1) Use 'units/mg solid' for your calculations | ||
::2) Result: 40 mg catalase powder (2800 | ::2) Result: 40 mg catalase powder (2800 U/mg) are dissolved in 1 ml MiR05 to obtain a catalase stock solution with 112.000 U/ml. | ||
Revision as of 15:47, 21 May 2012
- high-resolution terminology - matching measurements at high-resolution
Catalase
Description
Catalase catalyzes the decomposition of hydrogen peroxide to water and oxygen.
Abbreviation: Cat
MitoPedia topics:
Enzyme
Application in HRR
Cat: Catalase (from bovine liver,lyophilized powder, 2.000-5.000 units/mg protein); Sigma C 9322, store at -20 °C
Preparation of catalase stock solution
- Stock solution: 112.000 u/ml (dissolved in MiR05)
- Example: 'Catalase lypophilized powder, 2800 units/mg solid and 3500 units/mg protein'
- 1) Use 'units/mg solid' for your calculations
- 2) Result: 40 mg catalase powder (2800 U/mg) are dissolved in 1 ml MiR05 to obtain a catalase stock solution with 112.000 U/ml.
Unit definition: * Units of enzymatic activitiy (u) in µmol/min; assay used by Sigma Aldrich: ' One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240. '
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 5 µl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 280 IU/ml
