Difference between revisions of "Catalase"
From Bioblast
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=Ctl | ||
|description='''Catalase''' catalyzes the decomposition of hydrogen peroxide to water and oxygen. | |description='''Catalase''' catalyzes the decomposition of hydrogen peroxide to water and oxygen. | ||
|info= | |info=[[MiPNet09.12]] | ||
|type=Enzyme | |type=Enzyme | ||
}} | |||
{{MitoPedia methods|type=Enzyme | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
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|type=Enzyme | |type=Enzyme | ||
}} | }} | ||
== Application in [[HRR]] == | == Application in [[HRR]] == | ||
''' | '''Clt: Catalase''' (from bovine liver,lyophilized powder, 2.000-5.000 units/mg protein); Sigma C 9322, store at -20 °C | ||
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'''Oxygraph-2k | '''Oxygraph-2k manual titrations:''' [[MiPNet09.12]] | ||
::* Titration volume: 5 µl using a 25 µl syringe | ::* Titration volume: 5 µl Ctl using a 25 µl syringe | ||
::* Final conc. in 2 ml O2k-chamber: 280 | ::* Final conc. in 2 ml O2k-chamber: 280 u/ml | ||
Revision as of 12:02, 29 August 2012
- high-resolution terminology - matching measurements at high-resolution
Catalase
Description
Catalase catalyzes the decomposition of hydrogen peroxide to water and oxygen.
Abbreviation: Ctl
Reference: MiPNet09.12
MitoPedia topics: Enzyme
Application in HRR
Clt: Catalase (from bovine liver,lyophilized powder, 2.000-5.000 units/mg protein); Sigma C 9322, store at -20 °C
Preparation of catalase stock solution
- Stock solution: 112.000 u/ml (dissolved in MiR05)
- Example: 'Catalase lypophilized powder, 2800 units/mg solid and 3500 units/mg protein'
- 1) Use 'units/mg solid' for your calculations
- 2) Result: 40 mg catalase powder (2800 U/mg) are dissolved in 1 ml MiR05 to obtain a catalase stock solution with 112.000 U/ml.
Unit definition: * Units of enzymatic activitiy (u) in µmol/min; assay used by Sigma Aldrich: ' One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240. '
Oxygraph-2k manual titrations: MiPNet09.12
- Titration volume: 5 µl Ctl using a 25 µl syringe
- Final conc. in 2 ml O2k-chamber: 280 u/ml
