Chance 1961 J Biol Chem-IV

Chance B, Hollunger G (1961) The interaction of energy and electron transfer reactions in mitochondria IV. The pathway of electron transfer. J Biol Chem 236:1562-8.

» PMID: 13692279 Open Access

Chance B, Hollunger G (1961) J Biol Chem

Abstract:

1. The pathway of electron transfer from succinate to pyridine nucleotide shows a sensitivity to antimycin A, suggesting that carriers of the respiratory chain up to and including the antimycin-sensitive point are involved in succinate-linked reduction of pyridine nucleotide.
2. The sensitivity of succinate-linked reduction of pyridine nucleotide to Amytal suggests that a reverse of the flavoprotein-pyridine nucleotide interaction observed in the oxidation of pyridine nucleotide in phosphorylating mitochondria is also part of the electron transfer pathway.
3. Mechanisms indicating the interconnection of electrons from the antimycin-sensitive point to this flavoprotein via electron carriers such as cytochrome b and ubiquinone are considered. These mechanisms appear to apply to both aerobic and anaerobic (terminally inhibited) energy-linked reduction of pyridine nucleotide.
4. Three mechanisms for increased reduction of pyridine nucleotide in succinate-treated mitochondria that do not involve the above pathway fail to show responses of the experimentally observed sensitivity to Amytal or to antimycin A.
5. The properties of the energy-linked pool of pyridine nucleotide in metabolism are considered. Its participation is likely to be small in state 3 and of some consequence in state 4.

• Keywords: Electron transfer, Succinate, Pyridine nucleotide, Antimycin A

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Organism: Guinea pig, Birds  Tissue;cell: Heart, Liver, Kidney  Preparation: Isolated mitochondria  Enzyme: Complex II;succinate dehydrogenase, Complex III 

Coupling state: OXPHOS 

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