Chance 1961 J Biol Chem-VI

Chance B, Hollunger G (1961) The interaction of energy and electron transfer reactions in mitochondria VI. The efficiency of the reaction. J Biol Chem 236:1577-84.

» PMID: 13692280 Open Access

Chance B, Hollunger G (1961) J Biol Chem

Abstract: The stoichiometry and efficiency of the interaction of adenosine 5’-triphosphate with the respiratory carriers has been evaluated. From oxidative recovery data, the adenosine 5’-triphosphate-electron ratio is about 1. Values from 1.0 to 1.5 are obtained from the direct effects of adenosine 5’-triphosphate in both the reduction of pyridine nucleotide and the oxidation of cytochrome, although the computations indicating a low stoichiometric ratio for oxidizing equivalents are based upon more assumptions than in the case of reducing equivalents. There is rough agreement in the number of oxidizing equivalents found in cytochrome and the number of reducing equivalents found in pyridine nucleotide, provided succinate is absent. The reversal of electron transfer occurs with the expenditure of a remarkably small amount of energy (fifteen thousand calories) in cytochrome oxidation and pyridine nucleotide reduction in the respiratory chain. An interpretation of such data suggests that the principal point of entry of adenosine 5’-triphosphate may be at the pyridine nucleotide-flavin couple, but the entry of adenosine 5’-triphosphate simultaneously at other points is also consistent with these data. • Keywords: ATP, Pyridine nucleotide, Cytochrome oxidation, Electron transfer, Energy efficiency

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Organism: Guinea pig  Tissue;cell: Heart, Liver, Kidney  Preparation: Isolated mitochondria  Enzyme: Complex II;succinate dehydrogenase, Complex V;ATP synthase  Regulation: ADP, Coupling efficiency;uncoupling  Coupling state: OXPHOS 

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