Difference between revisions of "Charles 2020 Nanomedicine (Lond)"
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|area=Respiration | |area=Respiration, mt-Structure;fission;fusion, Pharmacology;toxicology | ||
|instruments=Oxygraph-2k | |organism=Human | ||
|additional=2020-12 | |tissues=Endothelial;epithelial;mesothelial cell | ||
|preparations=Isolated mitochondria | |||
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|couplingstates=LEAK, OXPHOS | |||
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Revision as of 17:21, 2 December 2020
Charles C, Cohen-Erez I, Kazaoka B, Melnikov O, Stein DE, Sensenig R, Rapaport H, Orynbayeva Z (2020) Mitochondrial responses to organelle-specific drug delivering nanoparticles composed of polypeptide and peptide complexes. Nanomedicine (Lond) [Epub ahead of print]. |
Charles Carleigh, Cohen-Erez Ifat, Kazaoka Blake, Melnikov Olga, Stein David E, Sensenig Richard, Rapaport Hanna, Orynbayeva Zulfiya (2020) Nanomedicine (Lond)
Abstract: The mechanistic study of the drug carrier-target interactions of mitochondria-unique nanoparticles composed of polypeptide-peptide complexes (mPoP-NPs).
The isolated organelles were employed to address the direct effects of mPoP-NPs on dynamic structure and functional wellbeing of mitochondria. Mitochondria morphology, respiration, membrane potential, reactive oxygen species generation, were examined by confocal microscopy, flow cytometry and oxygraphy. Lonidamine-encapsulated formulation was assessed to evaluate the drug delivery capacity of the naive nanoparticles.
The mPoP-NPs do not alter mitochondria structure and performance upon docking to organelles, while successfully delivering drug that causes organelle dysfunction.
The study gives insight into interactions of mPoP-NPs with mitochondria and provides substantial support for consideration of designed nanoparticles as biocompatible and efficient mitochondria-targeted platforms. β’ Keywords: OxPhos, TPP+, Cancer, Ionidamine, Membrane potential, Mitochondria, Nanoparticles β’ Bioblast editor: Plangger M
Labels: MiParea: Respiration, mt-Structure;fission;fusion, Pharmacology;toxicology
Organism: Human
Tissue;cell: Endothelial;epithelial;mesothelial cell
Preparation: Isolated mitochondria
Regulation: mt-Membrane potential Coupling state: LEAK, OXPHOS Pathway: N, S, CIV, ROX HRR: Oxygraph-2k, O2k-Fluorometer, TPP
2020-12, AmR