Difference between revisions of "Chavanelle 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"
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|organism=Mouse | |organism=Mouse | ||
|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|preparations=Permeabilized tissue | |||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS | ||
|substratestates=FAO | |substratestates=FAO | ||
|additional=Palmitoylcarnitine | |additional=Palmitoylcarnitine | ||
}} | }} | ||
Revision as of 17:16, 11 November 2015
| Chavanelle 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Chavanelle V (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
Fatty oxidation in permeabilised db/db mice muscle fibres, in Mir05 + Cr +37°C. We found that ADP-stimulated respiration in presence of 0,02mM of palmitoyl-carnitine (OXPHOS) is very close to the non-ADP-stimulated respiration (LEAK state) when working with these conditions. (Values around 14 to 20 (pmol/s/mg w.t.) for LEAK state and 15 to 24 (pmol/s/mg w.t.) for fatty acid triggered-OXPHOS). These data suggest either a very poor ability for this animal model to use palmitoylcarnitine or a very high LEAK state (or both!)
Labels:
Organism: Mouse
Tissue;cell: Skeletal muscle
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS
Palmitoylcarnitine
