Difference between revisions of "Chinopoulos 2009"
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{{Publication | {{Publication | ||
|title=Chinopoulos C, Vajda S, Csanady L, Mandi M, Mathe K, Adam-Vizi V (2009) A Novel Kinetic Assay of Mitochondrial ATP-ADP Exchange Rate Mediated by the ANT. Biophys J 96, 2490-504. | |title=Chinopoulos C, Vajda S, Csanady L, Mandi M, Mathe K, Adam-Vizi V (2009) A Novel Kinetic Assay of Mitochondrial ATP-ADP Exchange Rate Mediated by the ANT. Biophys J 96, 2490-504. doi:10.1016/j.bpj.2008.12.3915 | ||
|info=[https://pubmed.ncbi.nlm.nih.gov/19289073/?from_single_result=A+Novel+Kinetic+Assay+of+Mitochondrial+ATP-ADP+Exchange+Rate+Mediated+by+the+ANT PMID: 24391134] | |info=[https://pubmed.ncbi.nlm.nih.gov/19289073/?from_single_result=A+Novel+Kinetic+Assay+of+Mitochondrial+ATP-ADP+Exchange+Rate+Mediated+by+the+ANT PMID: 24391134 Open Access] | ||
|authors=Chinopoulos Christos, Vajda Szilvia, Csanady Laszlo, Mandi Miklos, Mathe Katalin, Adam-Vizi Vera | |authors=Chinopoulos Christos, Vajda Szilvia, Csanady Laszlo, Mandi Miklos, Mathe Katalin, Adam-Vizi Vera | ||
|year=2009 | |year=2009 | ||
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|mipnetlab=HU Budapest Chinopoulos C | |mipnetlab=HU Budapest Chinopoulos C | ||
}} | }} | ||
::::» Fluorometry protocol for [[Magnesium green]] | |||
== Cited by == | == Cited by == | ||
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|additional=MgG, Safranin, ANT, MitoFit 2021 MgG | |additional=MgG, Safranin, ANT, MitoFit 2021 MgG | ||
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Latest revision as of 11:52, 9 February 2022
Chinopoulos C, Vajda S, Csanady L, Mandi M, Mathe K, Adam-Vizi V (2009) A Novel Kinetic Assay of Mitochondrial ATP-ADP Exchange Rate Mediated by the ANT. Biophys J 96, 2490-504. doi:10.1016/j.bpj.2008.12.3915 |
Chinopoulos Christos, Vajda Szilvia, Csanady Laszlo, Mandi Miklos, Mathe Katalin, Adam-Vizi Vera (2009) Biophys J
Abstract: A novel method exploiting the differential affinity of ADP and ATP to Mg(2+) was developed to measure mitochondrial ADP-ATP exchange rate. The rate of ATP appearing in the medium after addition of ADP to energized mitochondria, is calculated from the measured rate of change in free extramitochondrial [Mg2+] reported by the membrane-impermeable 5K+ salt of the Mg2+-sensitive fluorescent indicator, Magnesium Green, using standard binding equations. The assay is designed such that the adenine nucleotide translocase (ANT) is the sole mediator of changes in [Mg2+] in the extramitochondrial volume, as a result of ADP-ATP exchange. We also provide data on the dependence of ATP efflux rate within the 6.8-7.8 matrix pH range as a function of membrane potential. Finally, by comparing the ATP-ADP steady-state exchange rate to the amount of the ANT in rat brain synaptic, brain nonsynaptic, heart and liver mitochondria, we provide molecular turnover numbers for the known ANT isotypes.
• Keywords: Adenine nucleotide translocase, ANT, ATP-ADP exchange, Magnesium Green
• Bioblast editor: Cardoso Luiza HD,
• O2k-Network Lab: HU Budapest Chinopoulos C
- » Fluorometry protocol for Magnesium green
Cited by
- Cardoso et al (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. doi:10.26124/bec:2021-0001
Labels:
Organism: Rat
Tissue;cell: Heart, Nervous system, Liver
Preparation: Isolated mitochondria
Enzyme: Adenine nucleotide translocase
Regulation: ADP, ATP, ATP production
MgG, Safranin, ANT, MitoFit 2021 MgG