Chou 2015 Fatty Acid Oxidation O2k-Network Discussion Forum: Difference between revisions
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|year=2015 | |year=2015 | ||
|event=Fatty Acid Oxidation O2k-Network Discussion Forum | |event=Fatty Acid Oxidation O2k-Network Discussion Forum | ||
|abstract='''Protocol''': final concentration of digitonin in chamber is 10Ξg/ml | |||
cell number in chamber is 2 millions cell/ml, cell type PBMC, Malate (2mM), Palmitoyl-DLcarnitine-HCl (20ΞM), ADP (2.5mM), pyruvate (5mM), glutamate (10mM), succinate (10mM), rotenone (0.1ΞM), malonic acid(5mM), myxothiazol (0.5ΞM), antimycin A (2.5ΞM), TMPD (0.5mM), azide (100mM) | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|tissues=Blood cells | |||
|model cell lines=Lymphocyte, Platelet | |model cell lines=Lymphocyte, Platelet | ||
|preparations=Permeabilized cells | |preparations=Permeabilized cells, Permeabilized tissue | ||
|substratestates=FAO | |couplingstates=OXPHOS, ETS | ||
|substratestates=CI, CII, FAO, CI&II, ROX | |||
|additional=Palmitoylcarnitine | |additional=Palmitoylcarnitine | ||
}} | }} | ||
Revision as of 14:20, 2 December 2015
| Chou 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Chou Cheng-Hsien (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
Protocol: final concentration of digitonin in chamber is 10Ξg/ml cell number in chamber is 2 millions cell/ml, cell type PBMC, Malate (2mM), Palmitoyl-DLcarnitine-HCl (20ΞM), ADP (2.5mM), pyruvate (5mM), glutamate (10mM), succinate (10mM), rotenone (0.1ΞM), malonic acid(5mM), myxothiazol (0.5ΞM), antimycin A (2.5ΞM), TMPD (0.5mM), azide (100mM)
Labels:
Tissue;cell: Blood cells Preparation: Permeabilized cells, Permeabilized tissue
Coupling state: OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
Palmitoylcarnitine
