Difference between revisions of "Chou 2015 Fatty Acid Oxidation O2k-Network Discussion Forum"
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|abstract='''Protocol''': final concentration of digitonin in chamber is 10μg/ml | |abstract='''Protocol''': final concentration of digitonin in chamber is 10μg/ml | ||
cell number in chamber is 2 millions cell/ml, cell type PBMC, Malate (2mM), Palmitoyl-DLcarnitine-HCl (20μM), ADP (2.5mM), pyruvate (5mM), glutamate (10mM), succinate (10mM), rotenone (0.1μM), malonic acid(5mM), myxothiazol (0.5μM), antimycin A (2.5μM), TMPD (0.5mM), azide (100mM) | cell number in chamber is 2 millions cell/ml, cell type PBMC, Malate (2mM), Palmitoyl-DLcarnitine-HCl (20μM), ADP (2.5mM), pyruvate (5mM), glutamate (10mM), succinate (10mM), rotenone (0.1μM), malonic acid(5mM), myxothiazol (0.5μM), antimycin A (2.5μM), TMPD (0.5mM), azide (100mM) | ||
|keywords=PBMC | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|tissues=Blood cells | |tissues=Blood cells, Lymphocyte, Platelet | ||
|preparations=Permeabilized cells, Permeabilized tissue | |preparations=Permeabilized cells, Permeabilized tissue | ||
|couplingstates=OXPHOS, | |couplingstates=OXPHOS, ET | ||
| | |pathways=F, N, S, NS, ROX | ||
|additional=Palmitoylcarnitine | |additional=Palmitoylcarnitine, TW, | ||
}} | }} | ||
Latest revision as of 17:33, 25 February 2020
| Chou 2015 Fatty Acid Oxidation O2k-Network Discussion Forum |
Link: O2k-Network Discussion Forum
Chou Cheng-Hsien (2015)
Event: Fatty Acid Oxidation O2k-Network Discussion Forum
Protocol: final concentration of digitonin in chamber is 10μg/ml cell number in chamber is 2 millions cell/ml, cell type PBMC, Malate (2mM), Palmitoyl-DLcarnitine-HCl (20μM), ADP (2.5mM), pyruvate (5mM), glutamate (10mM), succinate (10mM), rotenone (0.1μM), malonic acid(5mM), myxothiazol (0.5μM), antimycin A (2.5μM), TMPD (0.5mM), azide (100mM)
• Keywords: PBMC
Labels:
Tissue;cell: Blood cells, Lymphocyte, Platelet Preparation: Permeabilized cells, Permeabilized tissue
Coupling state: OXPHOS, ET
Pathway: F, N, S, NS, ROX
Palmitoylcarnitine, TW
