Difference between revisions of "Christen 2013 Abstract MiP2013"
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{{Abstract | {{Abstract | ||
|title=Christen F, Desrosiers V, Blier PU (2013) Thermal sensitivity of mitochondria from arctic charr heart: Mitochondrial respiration and ROS production. Mitochondr Physiol Network 18.08. | |title=Christen F, Desrosiers V, Blier PU (2013) Thermal sensitivity of mitochondria from arctic charr heart: Mitochondrial respiration and ROS production. Mitochondr Physiol Network 18.08. | ||
|info=[[File:Logo MiP2013.jpg|150px|right|MiPsociety]][ | |info=[[File:Logo MiP2013.jpg|150px|right|MiPsociety]][[MiP2013]], [[Laner 2013 Mitochondr Physiol Network MiP2013|Book of Abstracts Open Access]] | ||
|authors=Christen F, Desrosiers V, Blier PU | |authors=Christen F, Desrosiers V, Blier PU | ||
|year=2013 | |year=2013 | ||
|event= | |event=MiPNet18.08_MiP2013 | ||
|abstract=In the context of climate change, it is of paramount importance to investigate the thermal sensitivity of aquatic ectoterms [1]. Oxidative phosphorylation in mitochondria is one of the key processes of energy production and is known to be influenced by temperature [2,3]. The aim of our study was to shed light on the specific steps of the electron transfer | |abstract=In the context of climate change, it is of paramount importance to investigate the thermal sensitivity of aquatic ectoterms [1]. Oxidative phosphorylation in mitochondria is one of the key processes of energy production and is known to be influenced by temperature [2,3]. The aim of our study was to shed light on the specific steps of the electron transfer-pathway ([[ET-pathway]]) that contribute to the adaptation of fish to temperature changes. For this purpose, we measured oxygen consumption and hydrogen peroxide production at four different temperatures (10, 15, 20 and 25°C) in mitochondria isolated from arctic charr heart (''Salvelinus alpinus'') raised at 10 °C. Activities of citrate synthase and cytochrome ''c'' oxidase (CIV) were also measured at the same temperatures. Specifically, respiration rates of Complex I and Complex II in both coupled and uncoupled states were determined separately by adding either pyruvate, malate, ADP and FCCP for Complex I or succinate, ADP and FCCP for Complex II. Moreover, respiration rates were also measured in the presence of pyruvate+malate+succinate+ADP allowing the evaluation of Complexes I+II together. | ||
Our preliminary results showed that the Complex I and Complex II respiration rates (taken together) were higher when measured separately than when both complexes worked simultaneously. However, this difference was only significant at 15 °C. This may concomitantly occur with a higher reactive oxygen species production at elevated temperatures, and potentially a disruption of mitochondrial integrity. Subsequent analyses of hydrogen peroxide production, citrate synthase and CIV activity will give us further insights into the thermal sensitivity of arctic charr heart mitochondria. | Our preliminary results showed that the Complex I and Complex II respiration rates (taken together) were higher when measured separately than when both complexes worked simultaneously. However, this difference was only significant at 15 °C. This may concomitantly occur with a higher reactive oxygen species production at elevated temperatures, and potentially a disruption of mitochondrial integrity. Subsequent analyses of hydrogen peroxide production, citrate synthase and CIV activity will give us further insights into the thermal sensitivity of arctic charr heart mitochondria. | ||
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{{Labeling | {{Labeling | ||
|area=Respiration, Comparative MiP;environmental MiP | |area=Respiration, Comparative MiP;environmental MiP | ||
|organism=Other mammals | |injuries=Oxidative stress;RONS | ||
|organism=Other mammals, Fishes | |||
|tissues=Heart | |tissues=Heart | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|enzymes=Marker | |enzymes=Marker enzyme | ||
|topics=Inhibitor, Temperature | |topics=Inhibitor, Temperature | ||
|couplingstates=OXPHOS, | |couplingstates=OXPHOS, ET | ||
| | |pathways=N, S, CIV, NS, ROX | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=MiP2013, S04 | |additional=MiP2013, S04 | ||
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# Lemieux H, Tardif JC, Dutil JD, Blier PU (2010) Thermal sensitivity of cardiac mitochondrial metabolism in an ectothermic species from a cold environment, Atlantic wolffish (Anarhichas lupus). J Exp Mar Biol Ecol 384: 113–118. | # Lemieux H, Tardif JC, Dutil JD, Blier PU (2010) Thermal sensitivity of cardiac mitochondrial metabolism in an ectothermic species from a cold environment, Atlantic wolffish (Anarhichas lupus). J Exp Mar Biol Ecol 384: 113–118. | ||
# Pichaud N, Chatelain EH, Ballard JWO, Tanguay R, Morrow G, Blier PU (2010) Thermal sensitivity of mitochondrial metabolism in two distinct mitotypes of ''Drosophila simulans'': evaluation of mitochondrial plasticity. J Exp Biol 213: 1665–1675. | # Pichaud N, Chatelain EH, Ballard JWO, Tanguay R, Morrow G, Blier PU (2010) Thermal sensitivity of mitochondrial metabolism in two distinct mitotypes of ''Drosophila simulans'': evaluation of mitochondrial plasticity. J Exp Biol 213: 1665–1675. | ||
Latest revision as of 11:16, 8 June 2020
Christen F, Desrosiers V, Blier PU (2013) Thermal sensitivity of mitochondria from arctic charr heart: Mitochondrial respiration and ROS production. Mitochondr Physiol Network 18.08. |
Link:
MiP2013, Book of Abstracts Open Access
Christen F, Desrosiers V, Blier PU (2013)
Event: MiPNet18.08_MiP2013
In the context of climate change, it is of paramount importance to investigate the thermal sensitivity of aquatic ectoterms [1]. Oxidative phosphorylation in mitochondria is one of the key processes of energy production and is known to be influenced by temperature [2,3]. The aim of our study was to shed light on the specific steps of the electron transfer-pathway (ET-pathway) that contribute to the adaptation of fish to temperature changes. For this purpose, we measured oxygen consumption and hydrogen peroxide production at four different temperatures (10, 15, 20 and 25°C) in mitochondria isolated from arctic charr heart (Salvelinus alpinus) raised at 10 °C. Activities of citrate synthase and cytochrome c oxidase (CIV) were also measured at the same temperatures. Specifically, respiration rates of Complex I and Complex II in both coupled and uncoupled states were determined separately by adding either pyruvate, malate, ADP and FCCP for Complex I or succinate, ADP and FCCP for Complex II. Moreover, respiration rates were also measured in the presence of pyruvate+malate+succinate+ADP allowing the evaluation of Complexes I+II together.
Our preliminary results showed that the Complex I and Complex II respiration rates (taken together) were higher when measured separately than when both complexes worked simultaneously. However, this difference was only significant at 15 °C. This may concomitantly occur with a higher reactive oxygen species production at elevated temperatures, and potentially a disruption of mitochondrial integrity. Subsequent analyses of hydrogen peroxide production, citrate synthase and CIV activity will give us further insights into the thermal sensitivity of arctic charr heart mitochondria.
• Keywords: Arctic charr, Topics:Effects of temperature
• O2k-Network Lab: CA Rimouski Blier PU
Labels: MiParea: Respiration, Comparative MiP;environmental MiP
Stress:Oxidative stress;RONS Organism: Other mammals, Fishes Tissue;cell: Heart Preparation: Isolated mitochondria Enzyme: Marker enzyme Regulation: Inhibitor, Temperature Coupling state: OXPHOS, ET Pathway: N, S, CIV, NS, ROX HRR: Oxygraph-2k
MiP2013, S04
Affiliations and author contributions
Dept de biologie, Université du Québec à Rimouski, Canada. - Email: christenfelix@gmail.com
References
- Pörtner HO, Knust R (2007) Climate change affects marine fishes through the oxygen limitation of thermal tolerance. Science 315: 95.
- Lemieux H, Tardif JC, Dutil JD, Blier PU (2010) Thermal sensitivity of cardiac mitochondrial metabolism in an ectothermic species from a cold environment, Atlantic wolffish (Anarhichas lupus). J Exp Mar Biol Ecol 384: 113–118.
- Pichaud N, Chatelain EH, Ballard JWO, Tanguay R, Morrow G, Blier PU (2010) Thermal sensitivity of mitochondrial metabolism in two distinct mitotypes of Drosophila simulans: evaluation of mitochondrial plasticity. J Exp Biol 213: 1665–1675.