Difference between revisions of "Claude 1944 J Exp Med"
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{{Publication | {{Publication | ||
|title=Claude A (1944) The constitution of mitochondria and microsomes, and the distribution of nucleic acid in the cytoplasm of a leukemic cell. J Exp Med 80: 19-29. | |title=Claude A (1944) The constitution of mitochondria and microsomes, and the distribution of nucleic acid in the cytoplasm of a leukemic cell. J Exp Med 80: 19-29. | ||
|info=[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135449/pdf/19.pdf PMID: 19871395 Open Access] | |info=[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2135449/pdf/19.pdf PMID: 19871395 Open Access] | ||
|authors=Claude A | |authors=Claude A | ||
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|organism=Rat | |organism=Rat | ||
|preparations=Homogenate, Isolated Mitochondria | |preparations=Homogenate, Isolated Mitochondria | ||
|additional=Made history | |||
}} | }} | ||
Revision as of 15:06, 13 June 2012
| Claude A (1944) The constitution of mitochondria and microsomes, and the distribution of nucleic acid in the cytoplasm of a leukemic cell. J Exp Med 80: 19-29. |
Abstract:
- Rat tumor extracts, containing chiefly the cytoplasmic constituents of leukemic cells, were fractionated into three main portions, the different components separating in the centrifuge according to size.
- Mitochondria were isolated by centrifugation at relatively low speed. Elementary composition of purified mitochondria was found to correspond to about 11.5 per cent nitrogen, 1.6 per cent phosphorus, and 27 per cent lipids. Phosphorus and nitrogen content of the lipid portion suggests that as much as 75 to 80 per cent of the lipids of mitochondria is represented by phospholipids. Tests for ribose nucleic acid were positive.
- Microsomes were separated by means of centrifugation at 18,000 x g. A relation between the high phosphorus content of the microsomes and the marked basophilia of the cytoplasm of leukemic cells is suggested.
- Phosphorus distribution in the tumor extract, and light absorption analysis of the third fraction, seem to demonstrate that nucleic acid was not present either in a free condition, or in the form of nucleoprotein of relatively low molecular weight. The nature of the results suggests that ribose nucleic acid occurs in the cytoplasm of leukemic cells only in association with formed elements of relatively large size, namely microsomes, and mitochondria.
• Keywords: mitichondria, microsomes, nucleic acid distribution, leukemic cells.
Labels:
Stress:Cancer; Apoptosis; Cytochrome c"Cancer; Apoptosis; Cytochrome c" is not in the list (Cell death, Cryopreservation, Ischemia-reperfusion, Permeability transition, Oxidative stress;RONS, Temperature, Hypoxia, Mitochondrial disease) of allowed values for the "Stress" property. Organism: Rat
Preparation: Homogenate, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Made history
