Difference between revisions of "Crabtree effect"
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Summary: 'The general result of these observations is to emphasise the difficulty of including the wide variations found in the carbohydrate metabolism of tumour tissue in one generalisation. The constant factor is the possession of a high aerobic glycolysis, which, though not specific for tumour tissue, is a source of energy available for uncontrolled proliferation.' | Summary: 'The general result of these observations is to emphasise the difficulty of including the wide variations found in the carbohydrate metabolism of tumour tissue in one generalisation. The constant factor is the possession of a high aerobic glycolysis, which, though not specific for tumour tissue, is a source of energy available for uncontrolled proliferation.' | ||
[[Crabtree 1929 Biochem J]] | [[Crabtree 1929 Biochem J|Crabtree HG (1929) Observations on the carbohydrate metabolism of tumours. Biochem J 23: 536–545.]] | ||
== Gnaiger (1990) == | == Gnaiger (1990) == | ||
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'At high fructose concentrations, respiration is inhibited while glycolytic end products accumulate, a phenomenon known as the Crabtree effect. It is commonly believed that this effect is restricted to microbial and tumour cells with uniquely high glycolytic capacities (Sussman et al, 1980). However, inhibition of respiration and increase of lactate production are observed under aerobic conditions in beating rat heart cell cultures (Frelin et al, 1974) and in isolated rat lung cells (Ayuso-Parrilla et al, 1978). Thus, the same general mechanisms responsible for the integration of respiration and glycolysis in tumour cells (Sussman et al, 1980) appear to be operating to some extent in several isolated mammalian cells.' | 'At high fructose concentrations, respiration is inhibited while glycolytic end products accumulate, a phenomenon known as the Crabtree effect. It is commonly believed that this effect is restricted to microbial and tumour cells with uniquely high glycolytic capacities (Sussman et al, 1980). However, inhibition of respiration and increase of lactate production are observed under aerobic conditions in beating rat heart cell cultures (Frelin et al, 1974) and in isolated rat lung cells (Ayuso-Parrilla et al, 1978). Thus, the same general mechanisms responsible for the integration of respiration and glycolysis in tumour cells (Sussman et al, 1980) appear to be operating to some extent in several isolated mammalian cells.' | ||
[[Gnaiger_1990_Biochim Biophys Acta|Gnaiger | [[Gnaiger_1990_Biochim Biophys Acta|Gnaiger E, Kemp RB (1990) Anaerobic metabolism in aerobic mammalian cells: information from the ratio of calorimetric heat flux and respirometric oxygen flux. Biochim Biophys Acta 1016: 328-332.]] | ||
Revision as of 11:02, 11 December 2012
- high-resolution terminology - matching measurements at high-resolution
Crabtree effect
Description
The Crabtree effect describes the observation that respiration is frequently inhibited when high concentrations of glucose or fructose are added to the culture medium - a phenomenon observed in numerous cell types, particularly in proliferating cells, not only in tumor cells, in bacteria, and yeast. The Pasteur effect (suppression of glycolysis by oygen) is the converse of the Crabtree effect (aerobic glycolysis to lactate or ethanol).
Abbreviation: n.a.
Reference: Crabtree 1929 Biochem J
MitoPedia methods:
Respirometry
Labels:
Preparation: Intact Cell; Cultured; Primary"Intact Cell; Cultured; Primary" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Aerobic and Anaerobic Metabolism"Aerobic and Anaerobic Metabolism" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
Crabtree (1929)
Introduction: 'Warburg has suggested several generalisations, showing characteristic relationships between the magnitudes of the respiration and the aerobic and anaerobic glycolysis. The constant result which emerged was the abnormally high value of the anaerobic glycolysis as compared with the respiration. Assuming that the oxygen utilised was functioning at its maximum efficiency in causing the removal or non-formation of lactic acid under aerobic conditions, the respiration was found inadequate to check the glycolysis completely, a relatively large excess fermentation remaining. ...
Summary: 'The general result of these observations is to emphasise the difficulty of including the wide variations found in the carbohydrate metabolism of tumour tissue in one generalisation. The constant factor is the possession of a high aerobic glycolysis, which, though not specific for tumour tissue, is a source of energy available for uncontrolled proliferation.'
Crabtree HG (1929) Observations on the carbohydrate metabolism of tumours. Biochem J 23: 536–545.
Gnaiger (1990)
'At high fructose concentrations, respiration is inhibited while glycolytic end products accumulate, a phenomenon known as the Crabtree effect. It is commonly believed that this effect is restricted to microbial and tumour cells with uniquely high glycolytic capacities (Sussman et al, 1980). However, inhibition of respiration and increase of lactate production are observed under aerobic conditions in beating rat heart cell cultures (Frelin et al, 1974) and in isolated rat lung cells (Ayuso-Parrilla et al, 1978). Thus, the same general mechanisms responsible for the integration of respiration and glycolysis in tumour cells (Sussman et al, 1980) appear to be operating to some extent in several isolated mammalian cells.'
Comment
Perhaps an extended definition is required compared to the Wikipedia definition of the Crabtree effect.
